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《CHINESE JOURNAL OF CHROMATOGRAPHY》 1997-04
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High Performance Liquid Chromatographic Determination of Reducing Sugars in Fruit Juices with Laser Resonance Raman Detection

Ding Mingyu (Department of Chemistry, Tsinghua University, Beijing, 100084) Koizumi Hitoshi and Suzuki Yoshihito (Department of Chemistry and Biotechnology, Yamanashi University, Yamanashi 400, Japan)  
A liquid chromatographic method using semi microcolumn separation combined with laser resonance Raman detection was developed for the determination of monosaccharides in fruit juices. Reducing sugars (galactose, glucose, mannose, arabinose, xylose and ribose) were derivatized with 4 dimethylaminoazobenzene 4′ sulfonyl (DABSYL) hydrazine. The derivatives of the monosaccharides exhibited strong resonance Raman scattering at 1136cm -1 when Ar + laser emission line 488.0nm was used. A semi microcolumn Inertsil ODS 2 (250mm×1.5mm i.d.) was used to the separation of the monosaccharide derivatives. The saccharides eluted within 25min. However the excess DABSYL hydrazine was retained strongly with a retention time of about 60min. In order to reduce the longer analysis time, the excess DABSYL hydrazine was removed by adding glyoxylic acid and then Na 2CO 3 aqueous solution when the derivation reaction was finished. As a result, the analysis time was reduced to about a half of its initial run time. The sensitivity of resonance Raman detection greater two orders of magnitude than refractive index (RI) detection. The detection limit of glucose is 10ng (5.5pmol). The high selectivity of Raman detection came from the facts that only the interested compounds in a given sample was derived with the Raman labeling reagent and that only the characteristic Raman bands of the derivatives were selectively detected. In this work, in spite of the coexisting large quantities of organic acids in fruit juice, the determined values of glucose agreed well with those obtained by RI detection without any derivatization procedure.
【CateGory Index】: O658/Q5
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【References】
Chinese Journal Full-text Database 6 Hits
1 Hu Jun; Hu Jiming (Center of Analysis and Measurement,Wuhan University,Wuhan 430072);Application Progress of Raman Techniques in Analytical Chemistry[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-06
2 XIE Jia-xing, Ru Zhan-jun, YU Zhu (Applied Sciences College, Harbin Univ. Sci. Tech., Harbin 150080, China);Contrast and Investigation of Mono-and Bi-triangle Sample Case on the Content of Milk Lactose with Laser Refraction[J];Journal of Harbin University of Science and Technology;2005-02
3 Wu Jianzhong Tang Shuze Sun Xi Peng Xichun Guo Kaiping (Department.of Food Science and Engineering,Jinan University,510632,China);Review on Detection Techniques about Primary Fruit Juice Content in Fruit Drinks[J];Food and Fermentation Industries;2006-02
4 CHEN Jian,XIAO Kai-jun,LIN Fu-lan (College of Light Industry and Food, South China University of Technology, Guangzhou 510640, China);Review on Raman Spectroscopy Application in Food Analysis[J];Food Science;2007-12
5 HAN Jian-xun1,2, CHEN Ying1*, HUANG Wen-sheng1, GE Yi-qiang2,3*, DONG Wen3 (1.Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100025; 2.College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 1000831; 3.China Rural Technology Development Center, Beijing 100045);Researching progress on authentication technology of apple juice[J];Food Science and Technology;2008-08
6 Zhou Jian\ Yang Bo\ Dai Xiuyu (Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080 China);DETECTION OF TREHALOSE IN TRANSGENIC TOBACCO BY HPLC WITH ELSD[J];Acta Microbiologica Sinica;2001-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIANG Hong, ZHAO Yu Ying, CUI Yan Jun, LIU Qin Xiang (Division of Natural Medicinal Chemistry, School of Pharmaceutical Sciences, Peking University, Beijing 100083);Flavonoids from the roots of Bupleurum chinense DC.[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;2000-03
2 MENG Xiu?xia, GAO Mang?lai(Chemical Engineering Department, Petroleum of University, Beijing 102249,China);Progress in Studying Carbon Nanotubes and Their Application[J];Journal of Binzhou Teachers College;2002-04
3 JIN Xue?yong1,DI Xue?jin1,ZHANG Nai?qiang2(1Linyi Teachers Co11eage,Linyi 276005,2Binzhou Teachers Colleage,Binzhou 256604,China);Development in Prepartion,Properties and Application of Room Temperature Ionic Liquid[J];Journal of Binzhou Teachers College;2002-04
4 YAO Ming lin, CHEN Xian zhong, ZHANG Zheng (Dept of Meas and Cont,Inst of Info Engin,Beijing University of Science & Technology,Beijing 100083,China);Ultrasonic liquid density sensor[J];Journal of Transducer Technology;2005-05
5 Liu Zhenqing (Tongji University, Shanghai 200092);ULTRASONIC VELOCITY METHOD FOR THE CONCENTRATION MEASUREMENTOF NITRIC ACID IN WATER[J];Journal of Test and Measurement Technology;1996-01
6 CHEN Li feng (Dept. of Chem. Eng., Dalian Inst. of Light Ind., Dalian 116034, China);Hydrolization Techwologe of Pueraria[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;1999-03
7 SHANG Peng,YANG Tie hong, JIA Min, MEI Qi bing, ZHU De sheng, ZHAO Wen ming,ZHAO De hua (Institute of Bioengineering, Xi'an Jiaotong University, Xi'an 710049, China);Inhibitory effects of polysaccharides of Angelica sinensis (Oliv.)Diels on murine transplanted tumors[J];Acta Academiae Medicine Militaris Tertiae;2001-11
8 WANG Xiao Juan, ZHU Ling Zhen, WANG Si Wang, WANG Jie Pin Institute of Materia Medical of Fourth Military Medical University, Xi’an 710033, China;Preparation process of optimizing Pingzhi capsules with orthogonal test[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-02
9 SHANG Peng 1, YANG Tie Hong 2, JIA Min 2,MEI Qi Bing 2, ZHAO Wen Ming 1, CAO Zhi Xian 3, ZHAO De Hua 2 1Institute of Bioengineering, Xi'an Jiaotong University, Xi'an 710049, 2Department of Pharmacology, Faculty of Preclinical Med;Purification and analysis of polysaccharides of Angelica sinensisi (Oliv.) Diels[J];Journal of The Fourth Military Medical University;2001-14
10 ZHAO Guo hong, LEI Xiao qiang, WANG Zhong lai, CHEN Li ren (Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000; China);Preparation of the Phosphate Modified Charcoal Self-assembled PLOT Capillary Column for Gas-Solid Chromatography[J];Analysis and Testing Technology and Instruments;2002-02
China Proceedings of conference Full-text Database 3 Hits
1 WuXin-ji Zhang Jin Wang Na Esamdin All Sun Zen-wen Wang Wei-xia Yusupu Aili ( National Astronomical Observatory, Chinese Academic of Science, Beijing 100012) ( The Department of Astronomy, Peking University, Beijing 100871);THE RECENT PROGRESS OF PULSAR OBSERVATION AT NAO OF CHINA[A];[C];2002
2 Li Guang-yu Ni Wei-Tou Tian Lan-lan ( Pueple Mountain Observatory, Chinese Academy of Sciences, 210008) ( National Astronomy Observatory, Chinese Academy of Sciences, 100012);PROGRESE OF DEVELOPING PMOE PLANETARY EPHEMERIS[A];[C];2002
3 Wang Hai-tao Zhou Bi-fang (National Astronomical Observatories of China, NIAOT, Nanjing 210042);KEY TECHNOLOGIES FOR OPTICAL APERTURE SYNTHESIS INTERFERENCE IMAGE AND MULTI - BEAM - INTERFEROGRAM SIMULATION[A];[C];2002
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Ming-jing1,ZHANG Wei1,ZHAO Dong-bao1,2 and LIU Xiu-hua1(1.College of Chemistry and Chemical Engineer,Henan University,Kaifeng 475001;2.Jiyuan Vocational and Technical College,Jiyuan 454650);Determination of the contents of total flavonoids in Artemisia ordosica by three wavelength spectrophotometry[J];Chinese Journal of Analysis Laboratory;2007-03
2 Li Guoliang1,Song Guangjun2,Yao Zhixiang3,Jian Huali4(1.College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004;2.The Group of Logistic Industry,South China University of Technology,Guangzhou 510640;3.Department of Biological Chemical Engineering,Guangxi University of Technology,Liuzhou 545006;4.College of Food Science,South China Agricultural University,Guangzhou 510642,China);Applications of Raman Spectrometer in Process Monitering[J];Guangdong Chemical Industry;2008-05
3 Zhu Ye-jin~1,Zhang Min-jie~2(1.Department of Scientific Research Gannan Teacher's Colloge;2.Department of Chemistry and Life science Gannan Teacher's Colloge,Ganzhou 341000,China);Development of HPLC-ELSD's Application in Medicament Analysis[J];Journal of Gannan Teacher's College;2003-06
4 DU Yong, FANG Yan*ty,BeiJing 100037,China);NIR-SERS Study on Crystal Violet Molecules at Acidic Conditions[J];Chinese Journal of Light Scattering;2003-03
5 CHEN Liu, ZHANG Guo-ping (Dept. of Electronic and Information Engineering, College of Physical Science and Technology, Central China Normal University, Wuhan 430079);The Applications of Raman Spectroscopy to Water Analyse: Prospect and System Design[J];Chinese Journal of Light Scattering;2004-02
6 KANG Xu-sheng1,3, LIU Li-ke1, LIU Gen-qi1, HOU Ning1, DAI Xiu-yu2, LIU Chun-guang1 (1.Insititute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;2.Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China;3.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China);Activity of Inducible Expression of Prd29A Promoter in Wheat and Transformation of Escherichia coli Trehalose-6-phosphate Synthase Gene[J];Journal of Agricultural Biotechnology;2007-03
7 Wang Jiu-hui1 Zhou De-gui2 Wu Hua-youg3 (1.The productive quality supervision and inspection office of FUZHOU city,334000;2.Jiangxi Health Yyijiayi Beverage Ltd.,Jiangxi Province,pingxiang 337000, China;3.Key Laboratory of Food Science,Ministry of Education,Nanchang University,Nanchang 330047,China);Determination of Original Juice Content in White Radish Juice[J];Jiangxi Chemical Industry;2008-03
8 SHAO Yi, YUAN Qin-sheng (State Key Lab of Bioreactor Engineering, East China University of Science & Technology , Shanghai 200237, China);Study on the most optimized process of extracting trehalose from dried baker yeast[J];Chinese Journal of Biochemical Pharmaceutics;2002-06
9 Ma Yaohong Shi Janguo Yang Junhui Zhang Liqun Yang Yan Men Qingjun 1(Chinese-Japan Friendship Biotechnology Research Center of Shandong Academy of Sciences,Jinan 250014,China) 2(Shandong University,Jinan 250010,China);Rapid Determination of Reducing Sugar in Fruit Juice and Drinks[J];Food and Fermentation Industries;2006-06
10 HU Zong-li 1 XIA Yu-xian 1,2 CHEN Guo-ping 1,2 CAI Shao-xi 2 (1 Genetic Engineering Research Center,Chongqing University 2 Bioengineering College, Key Laboratory of Biomechanics & Tissue Engineering Ministry of Education,Chongqing University,Chongqing 400030,China);The Manufacture and Applcations of Trehalose[J];Progress In Biotechnology;2004-04
China Proceedings of conference Full-text Database 1 Hits
1 CHEN Jie~(1,2) YE Xing-guo~(2*) HE Cong-fen~(1*) DU Li-pu~2 DONG Yin-mao~1 1.Key lab of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100037,China; 2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Genetic transformation of Aloe via microprojectile bombardment[A];[C];2005
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