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Purification and identification of polysaccharide from Lentinus edodes

Guo Yazhen, Wang Zao (College of Food Science, SFU, 200090) Qu Pengpeng (Laboratory of Food Package and Technology, Shanghai Food Research Institute, 200233)  
Water soluble polysaccharide (lentinan) was crudely extracted from Lentinus edodes, then purified by the methanol fractionation with polysaccharide solution: methanol(v/v) 1: 0. 5 and 1: 1 respectively named ben 1 and ho 2. subsequently, the deproteinized polysaccharide was refined by gel filtration with Sephadex G-100 named Len 3. The finished products were identified by paper chromatography and gel filtration with Sephadex G-100. The compositions of ten 2 and Len 3 were separated by paper-and gas-chromatography. The results show that Len 2 is composed of mannose and glucose with a molar ratio of 10. 50: 1. 00, but Len 3 is composed of xylose, mannose and glucose with a molar ratio of 0.67:2 .45: 1 .00.
【CateGory Index】: TS201.2
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