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《Food Science and Technology》 2012-04
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Preliminary on extraction and purification of cAMP from Chinese jujube

WANG Li,QI Shu-yan,MO Xiao-yan(School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049)  
Objective:To study and optimize the conditions of extraction and purification of cyclic adenosine monophosphate(cAMP) from Chinese jujube.Methods:The RP-HPLC method was used to mensurate the content of cAMP,and the conditions of extraction were optimized with single factor and orthogonal experiment,and the macroporous adsorptive resins were selected to purify cAMP based on adsorption and desorption tests.Results:The optimum extraction conditions for cAMP from jujube by water were solid-liquid ratio 1:3,90 ℃ for 4 h,and the extraction rate of cAMP reached 12.03 mg/kg fresh jujube.In addition the AB-8 was selected from five type of macroporous adsorption resins to purify cAMP,and the purity of cAMP attained 58.2% under the conditions of 15 μg/mL cAMP extract being added 64 mL with flow rate 1 BV/h,AB-8 adsorbing 24 h,30% ethanol eluanting 4 h at flow rate 0.5 BV/h.
【CateGory Index】: TQ461
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【Co-citations】
Chinese Journal Full-text Database 7 Hits
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