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《Food Science and Technology》 2012-06
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GeO_2-enriched culture and antioxidant activity of total flavonoids of Inonotus obliquus

HUANG Xiao-dong1,CAI Jing-xiu2*,XU Li-xuan3,ZHENG Yue-bo2(1.Institute of Wetland,Quanzhou Normal College,Quanzhou 362000;2.College of Chemistry and Life Science,Quanzhou Normal University,Quanzhou 362000;3.Quanzhou Preschool Education College,Quanzhou 362000)  
The suitable dose of GeO2 in culture medium was investigated by the index of Inonotus obliquus colony diameter,then total flavonoids of Inonotus obliquus from GeO2-enriched medium(Ge-TFIO) were extracted by the optimum microwave technic conditions from L9(34) orthogonal experiment,meanwhile its antioxidant activities were evaluted by the ROS reagent kits,DPPH model and the inhibition test on hemolysis of mice RBC(red blood cell) by H2O2-induced.The results showed that Inonotus obliquus were suitably growing in the culture medium added with 40 mg/L GeO2,its total flavonoids extracting rate was 4.59% while flavonoids were extracted by the conditions of 352 wattage microwave power,40 ratio of liquid to material and 180 seconds microwave treatment time.The results also showed that 2 mg/mL GeTFIO had 25.85 U/mL anti-ROS units,91.39% scavenging rate on DPPH radical,29.13% inhibitory rate on mice RBC hemolysis by H2O2-induced.Therefore,it can be clearly seen that total flavonoids of Inonotus obliquus from GeO2-enriched medium have higher yield and stronger antioxidant activities.
【Fund】: 福建省科技厅重点项目(2009N0049);; 泉州师范学院福建省高校服务海西建设重点项目(A101);泉州师范学院开放实验室项目(20110111);泉州师范学院大学生创新实验项目(2011CXSY校02);; 福建省教育厅科技项目(JA11352)
【CateGory Index】: TS201.3
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