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《Acta Hydrobiologica Sinica》 2001-04
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IMMUNOCYTOCHEMICAL IDENTIFICATION AND LOCALIZATION OF APUD CELLS OF CHINESE MITTEN-HANDED CRAB ON DIFFERENT DIVELOPED STAGES

FANG Zhi-ping 1, PAN Qian-sheng 2,ZHAO Ya-xin 1,HE Jie 2, HUANG Feng-jie 1 and LUO Li-ting 1 (1. College of Animal Husbandry and Veterinary Medicine,2. College of Fisheries, Huazhong Agricultural University,Wuhan 430070)  
Using 12 kinds of antisera raised against mammalian from insects,fishes and mammalian,the APUD (amine pricursor uptake and decarboxylation) cells in the body of the first-fifth zoeal stages (Z 1-Z 5), a megalopal stage(M),a larva stage (L) of Chinese mitten-handed crab (Eriocheir sinensis) and in the digestive system of it's adult stage were identified and localized by using immunocytochemical technique of the strept avidin biotin-peroxidase complex method. The results showed that the immunoreactive positive cells in the body of the Z 1-Z 5,M and L of E. Sinensis were not found;9 kinds of antisera immunoreaction of nerve fiber protein,insulin,vasoactive intestinal polypeptide,calcitonin,5-hydroxytriptamine,calcitonin gene related peptide,growth hormone,calmodulin and molting hormone were found in the different parts of digestive system of E. Sinensis adult except hindgut. However 3 antisera of immunoreactive positive reaction of glucagon,substance P and neuron specific enuolase were not found in the digestive system of E. Sinensis adult. In present paper,the distribution of the above 9 kinds of immunoreactive positive reaction in the digestire system and their characteristics were described emphatically.
【Fund】: 国家自然科学基金资助项目 ( 39770 581 ) ;; 湖北省自然科学基金资助项目 ( 97J0 60 )
【CateGory Index】: S968.25
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