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《Acta Hydrobiologica Sinica》 2008-03
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EFFECT OF MEDIUM pH ON POPULATION GROWTH,ASEXUAL REPRODUCTION AND ACTIVITY OF SIX ENZYMES OF DUGESIA JAPONICA

PAN Hong-Chun,FAN Jie,WANG Fang-Fang and SUN Zhen (Key Laboratory of Biological Macromolecular Evolution,College of Life Sciences,Anhui Normal University,Wuhu 241000)  
The planarian Dugesia japonica has a very wide distribution throughout fresh water body in China,especially in mountain streams.Because of the advantages of easy laboratory culture and fast asexual reproduction rate,Dugesia japonica is generally used as an experimental animal for cell biology, reproductive biology and stem cell as well as animal regeneration research.pH is one of the important factor affecting population dynamics,reproductive and other life activities of aquatic organisms,and is also the basic laboratory culture condition of planarian.However,there were no reports about the effect of pH on planarin asexual reproduction and population dynamics.The purpose of the present study was to make certain appropriate pH value for large-scale laboratory cultivation of the planarian.In addition,the study can provide the first-hand information for the in-depth research about planarian adaptability to the environment. With the method of population accumulation culture,the effect of medium pH on survival,population growth,asexual reproduction and the activities of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-PX),lactic dehydrogenase (LDH),Na+-K+-ATPase and Ca2+-Mg2+-ATPase of Dugesia japonica was observed.The result indicates that the threshold of pH is 3.0—11.0,the appropriate pH range is 4.5—8.5.pH had evident effects on planarian population growth,population density and population instantaneous growth rate varied obviously with different media pH.After 27 days,the planarian population increased positively at pH 4.0—9.0,and at pH 4.5—6.5,the planarian population density was the highest,at pH 7.5—8.5,it was higher,at pH 7.0 was lower,and at pH 4.0 and pH 9.0,it was the lowest;whereas,at pH 9.5 the planarian population increased negatively.Planarian at pH 7.0 is in very good condition (such as normal feeding and locomotion behavior,maintaining a certain asexual reproductive rate and zero mortality),but the asexual reproductive rate of planarian at pH 7.0 is lower than that at pH 4.5—6.5 and pH 7.5—8.5.It is possibility that the higher asexual reproduction rate of the planarians at pH 4.5—6.5 and pH 7.5—8.5 than that at pH 7.0 is the response of planarian to the intimidation of acidity or alkalinity, another possibility is that the moderate pH value for planarian survival is not consistent with one for asexual reproduction by transverse fission. When media pH departed from 7.0 (media pH becomes acid or alkali),the activities of CAT,GSH-PX,Na+-K+-ATPase and Ca2+-Mg2+-ATPase had the increasing trend,while the activities of SOD and LDH had the decreasing trend.That is to say,the activities of the planarian's six enzymes all changed evidently with the departure of media from pH 7.0.Especially,planarian population density in the range of pH 4.0—9.5 correlated positively with the level of CAT activity which is deemed as an important indicator of antioxidant level of organisms.For example,the formula of the regression relationship between CAT activity (Y) and planarian population density (X) at the 27th day is Y=0.706 X+1.567 (R2=0.614,p=0.003).Furthermore,the level of CAT activity at pH 4.5—6.5 and pH 7.5—8.5 is 3—8 times higher than that at pH 7.0.Inverse environment usually causes the raise of antioxidant enzyme level,and it was a hint that pH 7.0 is the most suitable pH value for planarian survival.
【Fund】: 安徽省“重要生物资源保护与利用研究”省级重点实验室专项基金;; 安徽省高校“生物环境与生态安全”省级重点实验室专项基金;; 安徽师范大学博士科研启动基金项目资助
【CateGory Index】: Q4
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