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《Acta Hydrobiologica Sinica》 2011-05
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SUN Dian-Qiao,SUN Yue-Na,WANG Ri-Xin and XU Tian-Jun(Laboratory for Marine Living Resources and Molecular Engineering,College of Marine Science,Zhejiang Ocean University,Zhoushan 316000,China)  
To explore large-scale and use the type I of microsatellite markers,microsatellite sequences in Miichthys miiuy genome transcription were analyzed and selected of polymorphism by establishing full homogenization cDNA library and random sequencing.Program of Tandem Repeat Finder(TRF) was used to select the sequences contained simple sequence repeats(SSR) from the expressed sequence tags(ESTs) of M.miiuy.Then some characterizations,such as the repeat type,time and number,the percentage of SSR and ESTs and total length,were added up based on the motif.Totally,382 sequences contained not less than 14 bp simple repeats were obtained from 4609 ESTs of M.miiuy in our study,about 8.29% in the total database and on average about 318 bp length of EST contained a repeat sequence not less than 14 bp.All of the SSR selected were low-copies and the repeat types were abundant,including dinucleotide,trinu-cleotide,four-nucleotide,five-nucleotide and six-nucleotide repeat.Among the kinds of microsatellite repeats,these types,dinucleotide and trinucleotide repeat were the most abundant and it was 37.43% and 32.98% of the total mi-crosatellite repeats,respectively.These types of microsatellites were 70.41% of the total,but the advantage motif type of each were different.The AC repeat was the most common type(75.4%) in dinucleotide repeats,while in trinucleotide repeats,the A and G combination motifs(AGG and AAG) was the most common,which was 44.75% of all motifs.At the same time,the initiative polymorphism was tested to examine the value of EST-SSR loci selected in our study.Fi-nally,33 pairs of primers were successfully amplified of 45 pairs of designed primers,and nine loci were polymorphism.The polymorphism loci were about 20% of the total primers.So the type of microsatellite we selected was great abun-dant and useful for the further study.The results provided the basis for comparing the frequency and abundance of the microsatellites distribution between species and the development of type I microsatellite markers in M.miiuy and some allied species.The nine polymorphism loci could be used in genetic diversity analysis,genome mapping and molecular assisted breeding in M.miiuy.
【Fund】: 国家自然科学基金项目(31001120);; 浙江省自然科学基金项目(Y3100013)资助
【CateGory Index】: S917.4
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2 SHAN Xiu-Juan, DOU Shuo-Zeng (Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071);ALLOMETRIC GROWTH OF CROAKER MIICHTHYS MIIUY LARVAE AND JUVENILES AND ITS ECOLOGICAL IMPLICATION[J];Oceanologia Et Limnologia Sinica;2009-06
3 SHAN Le-zhou,XIE Qi-lang,SHAO Xin-bin,YAN Mao-cang(Zhejiang Mariculture Reseach Institute,Wenzhou 325005,China);Study on embryonic development and morphological characteristic habitual behavior for larvae,juvenile and young of Miichthys Miiuy[J];Marine Sciences;2010-01
4 KONG Jie1 & GAO Huan2,3 1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; 3. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;Analysis of tandem repeats in the genome of Chinese shrimp Fenneropenaeus chinensis[J];科学通报(英文版);2005-14
5 XU Peng,ZHOU Linghua,TIAN Liping,XIANG Jianhai(Institute of Oceanology, Chinese Academy of Sciences, Qingdao266071, China);Isolation of microsatellite markers from Fenneropenaeus chinensis ESTs[J];Journal of Fisheries of China;2003-03
6 SHI Yao-hua1,2,3, HONG Kui1, GUO Xi-ming3,WANG Yan2, HOU Zhan-hui2, GU Zhi-feng2, WANG Ai-min2(1. South China University of Tropical Agriculture, Institute of Tropical Biotechnology, CATAS, National Key Laboratory of Tropical Crop Biotechnology, Haikou 571101, China;2. Ocean College, Hainan University, Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, Haikou 570228,China;3. Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Avenue, Port Norris, NJ 08349, USA);Microsatellite markers screening from EST sequences of Pinctada martensii Dunker[J];Journal of Fisheries of China;2008-02
7 WANG Chun-Yan1,2,YU Xiao-Mu1 and TONG Jin-Gou1(1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072;2.Graduate School of Chinese Academy of Sciences,Beijing 100039);DEVELOPMENT AND CHARACTERIZATION OF POLYMORPHIC EST MARKERS IN COMMON CARP[J];Acta Hydrobiologica Sinica;2008-02
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2 KUANG Gang-Qiao 1,2,LIU Zhen1,LU Shuang-Qing 1,LIU Hong-Yu3,ZHANG Jian-She1,XIAO Tiao-Yi21.Department of Biotechnology and Environment Science,Changsha University,Changsha 410003,Hunan Province,China2.College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,Hunan Province,China3.Department of Environmental Science and Engineering,Hunan University,Changsha 410082,Hunan Province,China;Isolation and characterization of microsatellite loci of Siniperca chuatsi from GenBank database[J];Acta Zoologica Sinica;2007-01
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7 JIANG Ye-qin (School of Life Sciences,Huzhou Teachers College,Huzhou 313000,China);Status quo of the study on the genetic diversity of shrimps[J];Journal of Marine Sciences;2009-01
8 CHANG Yu-Mei1, GAO Guo-Qiang1,2, XU Li-Hua1,2, LIU Jin-Liang1,2, LIU Chun-Lei1,2, LIANG Li-Qun1,* (1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China);Correlation and location of EST markers with cold tolerance trait of common carp (Cyprinus carpio L.)[J];Zoological Research;2010-06
9 GAO Huan1,YU Fei2,3,YAN Binlun1,CHEN Jianhua1,KONG Jie2*(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;2.Marine Fisheries Research Institute of Lianyungang,Lianyungang 222042;3.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China);ADVANCES OF MICROSATELLITE TECHNOLOGY AND ITS APPLICATION IN MARINE BIOLOGY[J];Transactions of Oceanology and Limnology;2008-04
10 LIU Ping, MENG Xian_Hong, HE Yu_Ying, KONG Jie, LI Jian, WANG Qing_Yin (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071);GENETIC DIVERSITY IN THREE WILD POPULATIONS OF SHRIMP FENNEROPENAEUS CHINENSIS IN YELLOW AND BOHAI SEAS AS REVEALED BY MICROSATELLITE DNA[J];Oceanologia Et Limnologia Sinica;2004-03
【Secondary Citations】
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1 ZHAO Ying-Ying 1,2, ZHU Xiao-Chen2, SUN Xiao-Wen 1**, LIANG Li-Qun1,SUN Yang2, LIU Dan21.Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070,China2.Life Science and Technology Institute, Dalian Fisheries University, Dalian 116023, Liaoning, China;Polymorphic microsatellite loci of the Japanese scallop Patinopecten yessoensis[J];Acta Zoologica Sinica;2006-01
2 WANG Yong\|Qing ① WANG Xin\|Guo ② XU Lai\|Xiang ① ZHANG Zhi\|Bin ① (①Institute of Zoology,Chinese Academy of Sciences\ Beijing\ 100080; ②Institute of Biological Engineering\ Beijing\ 100071, China);A New Rapid Method for Extraction of High Quality of Genomic DNA from Animal Tissues[J];Chinese Journal of Zoology;2001-01
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4 Huang Yongchun Zheng jianhui Zhou Zebin (Aquaculture Department,Xiamen Fisheries College,Jimei 361021);The Effects of Salinity on the Embryonic Development and Larval Survival of Nibea miichthicides[J];Journal of Fujian Fisheries;1997-01
5 Li Honglei, Song Linsheng, Wang Lingling, Xu Wei, Xiang Jianhai (Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071);Screening Microsatellite Markers from EST Sequence of Chlamys farreri[J];High Technology Letters;2003-12
6 HONG Wan-shu, ZHANG Qi-yong(Department of Oceanography, Xiamen University, Xiamen 361005, China);ARTIFICIAL PROPAGATION AND BREEDING OF MARINE FISH IN CHINA[J];中国海洋湖沼学报(英文版);2002-01
7 XU Peng,ZHOU Ling Hua,XIANG Jian Hai (Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071);ISOLATING MICROSATELLITE DNA OF CHINESE SHRIMP PENAEUS CHINENSIS[J];Oceanologia Et Limnologia Sinica;2001-03
8 GAO Huan, LIU Ping , MENG Xian-Hong , WANG Wei-Ji , KONG Jie (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071; Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071; Graduate School, Chinese Academy of Sciences, Beijing, 100039)  (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071);ANALYSIS OF MICROSATELLITE SEQUENCES IN CHINESE SHRIMP FENNEROPENAEUS CHINENSIS GENOME[J];Oceanologia Et Limnologia Sinica;2004-05
9 LIU Bi-Qian, ZENG Qing-Guo, LUO Qi-Jun, WANG Ya-Jun, LI Sheng-Hua (Key Lab of Marine Biotechnology, Ningbo University, Ningbo, 315211) (The Institute of Ocean and Fishery of Ningbo, Ningbo, 315012);ISOLATION OF MICROSATELLITE LOCI FROM dbEST OF ALGAE PORPHYRA YEZOENSIS AND PRIMER AMPLIFICATION OF INTERSPECIES TRANSFER[J];Oceanologia Et Limnologia Sinica;2005-03
10 SHAN Xiu-Juan~(1,2) DOU Shuo-Zeng (Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071 2.Graduate school,Chinese Academy of Sciences,Beijing,100049);EFFECTS OF DELAYED FIRST FEEDING ON GROWTH,SURVIVAL AND BIOCHEMICAL COMPOSITION OF CROAKER MIICHTHYS MIIUY LARVAE[J];Oceanologia Et Limnologia Sinica;2008-01
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