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《Ecologic Science》 2006-06
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Study on the susceptibility tests of antibiotics and obtain axenic culture of Nannochloropsis ocutala

LIU Xiao-juan, DUAN Shun-shan(Institute of Hydrobiology, Jinan University, Guangzhou 510632, China)  
Effects of 5 antibiotics (chloramphenicol, penicillin, streptomycin, kanamycin sulfate, gentamycini sulfate) at different concentrations on the growth of Nannochloropsis ocutala were studied. Results showed that at 50 μg·mL-1, growth of N. ocutala was significantly inhibited by chloramphenicol, the highest inhibition was 85.30%, Ic50 of 2 d and 4 d was 293.64 μg·mL-1 and 67.69 μg·mL-1, respectively. Followed by penicillin, gentamycini sulfate, and kanamycin sulfate, the inhibition rate of 4 d was 8.95%, 7.62% and 3.84%, respectively. And 2 d Ic50 of gentamycini sulfate was 316.40 μg·mL-1. Growth of N. ocutala was not inhibited significantly by streptomycin, the inhibition rate was only 3%. According to susceptibility tests of antibiotics, a strategy of serial antibiotic treatments to obtain axenic culture was carried out: 50 μg·mL-1 penicillin,50 μg·mL-1 gentamycini sulfate,100 μg·mL-1 kanamycin sulphate, 50 μg·mL-1 streptomycin.
【Fund】: 国家自然科学基金资助(30370231);; 珠海市科技计划重大项目(PB20041018);; 广东省科技计划重点引导项目(2005B33201001)
【CateGory Index】: Q943.1
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