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《Ecologic Science》 2007-02
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Effects of antibiotics on the growth of Isochrysis zhangjiangensis and axenic culture

HUANG Zhen-hua, LIU Xiao-juan, HU Zhang-xi, DUAN Shun-shan(Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China)  
Effects of 5 kinds of antibiotics (chloramphenicol, ampicillin, streptomycin, kanamycin, gentamycin) at different concentrations on the growth of Isochrysis zhangjiangensis were studied, the axenic culture was obtained by mixed antibiotics. The results showed that: (1) There were differences among different antibiotics, chloramphenicol was the most susceptible antibiotic, the inhibition rate was 27.02% at the concentration of 50 μg·mL-1, more than 50 μg·mL-1 the inhibition rate was 80%. I.zhangjiangensis was not susceptible to ampicillin, streptomycin, kanamycin and gentamycin, there was stimulation at the lower concentration of the last three antibiotics. (2) Except for ampicillin at the concentration of 50 μg·mL-1, there were bacteriostasis effects of the other treatments. (3) Mixed antibiotics (500 μg·mL-1streptomycin,1000 μg·mL-1 kanamycin, and 50 μg·mL-1 gentamycin) were added to the culture medium by 4 combinations, the axenic culture was obtained, and there were stimulation on the growth of I.zhangjiangensis.
【Fund】: 国家自然科学基金(30370231);; 广东省科技计划重点引导项目(2005B33201001);; 珠海市科技计划重大项目(PB20041018)
【CateGory Index】: Q943.1
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