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《Chinese Journal of Ecology》 2018-09
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Genotoxicity of rare-earth element cerium on zebrafish( Danio rerio ) livers

HE Yan-bin;TAI Pei-dong;SUN Li-zong;GUO Cheng;LIU Zhi-hong;CONG Shi-long;Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology, Chinese Academy of Sciences;University of Chinese Academy of Sciences;Liaoning Shihua University;Deakin University;  
Cerium( Ce) is the most abundant rare-earth element. Using RAPD-PCR( random amplified polymorphic DNA-PCR) and MSAP-PCR( methylation-sensitive amplified polymorphism-PCR),the effects of Ce on genomic DNA damage and DNA methylation in zebrafish( Danio rerio) were examined. The results showed that,after the fish was exposed to different concentrations of Ce~(3+)( 0,5,10 and 20 μmol·L~(-1)) for 28 days,Ce~(3+)was mainly enriched in the liver( bio-concentration factors BCF: 5.49-9.33),followed by gill( BCF: 3.58-4.49) and muscle( BCF: 0.13-0.25). The results of RAPD-PCR showed that Ce~(3+)( 10 μmol·L~(-1)) could induce DNA damage in zebrafish livers. The results of MSAP-PCR showed that the change rates of total genomic DNA methylation of zebrafish livers caused by Ce~(3+)stress were 8. 93%( CK),9.12%( 5 μmol·L~(-1)),15.56%( 10 μmol·L~(-1)),and 28.83%( 20 μmol·L~(-1)),respectively.The demethylation( D) of genomic DNA in zebrafish livers significantly increased under the low concentration of Ce~(3+),while the methylation( M) significantly increased under the high concentration of Ce~(3+). Meanwhile,the methylation polymorphism increased with the increases of Ce~(3+)concentration. Results from sequencing analysis showed that these specific bands had high homology with the genes of zinc finger protein STZ/ZAT10,ABC transporter 1,and cell cycle control phosphatase superfamily protein. Compared to RAPD markers,DNA methylation markers were highly sensitive to Ce~(3+)stress. These results contribute to our understanding of the biological effects of rare earth elements.
【Fund】: 国家重点研发计划项目(2016YFD0800305);; 国家自然科学基金项目(21677151、41673132、31600372);; 辽宁省自然科学基金项目(201601329)资助
【CateGory Index】: X171.5
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