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《Progress in Modern Biomedicine》 2008-03
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The effect of static high magnetic field on bacillus subtilis

YIN Huan-cai XUE Xiao=ping TANG Rui-hua XIE Yu-wei SU Jing SONG Kai College of Life Sciences,Northwestern Polytechnic University,Xi'an,Shanxi,710072,China  
Objective:To study the effect of static high magnetic field(SHMF)on the microorganism.Methods:Based on the SHMF produced by superconducting magnets,bacillus subtilis being regarded as model organism,the characters of the microorganism were studied in the SHMF by determining the growth curves,growth rate of spores,expression and activity of proteases and so on. Results:Static high magnetic field could affect the forming rate of spores and reduce the death rate of vegetative mass.Through detecting the proteases content,alkaline and neutral proteases in the growth process of germs,it was discovered that there was no significant change in the content of proteases before/alter magnetic treatment;the enzyme activity of alkaline proteinase in the treatment group was obviously higher than that in the control group,but the enzyme activity of neutral proteinase lower than that in the control group. Conclusions:The static high magnetic field can prolong the generation cycle of bacilli subtilis and reduce the death rate of germs,which varies with the species of enzymes.
【Key Words】: Superconducting magnets Static high magnetic field Bacillus subtilis Protease
【CateGory Index】: Q937
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