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《Progress in Modern Biomedicine》 2011-04
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Effect of 2,3,5,4'-tetrahydroxystibene-2-o-β-D-glucoside in mitigation of MPP+-induced Apoptosis of PC12 Cells

TAO Li-zhen1,LI Yan2,LI Xiao-feng1,LI Xiao-bing1,SUN Xin1,CHEN Jian-zong1(1 Research Center of Traditional Chinese Medicine,Xijing Hospital,Fourth Military Medical University,Xi'an 710032; 2 Pharmaceutical Preparation Section,Xijing Hospital,Fourth Military Medical University,Xi'an 710032)  
Objective:To explore the protective effect of TSG on MPP+ induced PC12 cells apoptosis.Methods:The PC12 cells viability was measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyhetrazolium bromide(MTT).The morphological change of PC12 cells was observed by Hoechst 333258 staining.The expression level of NF-κB(P65) and IκBα protein was detected by Western blotting.Results:Compared with the control group,the ratio of cell survival decreased(53.3±3.4%)(P0.01);cyto-chromatin concentration;nu-cleus showing strong staining after MPP+ treatment with PC12 cells for 24h.The ratio of cell survival increased(60.8±1.9%),(70.1±1.8%)(P0.01),(81.2±1.9%)(P0.01)after TSG(1,5,10μmol/L) pretreatment with PC12 cells for 24h;cyto-chromatin concentration re-duced and show the relation between quantity and result.Moreover,MPP+ is able to increase the expression level of NF-κB(P65)protein in the nucleus,decrease the expression level of IκBα protein in the cytoplasm of PC12 cells.The high expression level of NF-κB(P65)protein in the nucleus is decreased and the low expression level of IκBα protein in the cytoplasm of PC12 cells is increased after TSG pretreatment with PC12 cells,compared with MPP+ treatment group.Conclusion:TSG plays a possible neuroprotective role in MPP+-in-duced apoptosis of PC12 cells by a concentration-dependent manner,and the mechanism of the effects of TSG may be involved in inhi-biting NF-κB activation.
【Fund】: 国家自然科学基金(30772743)
【CateGory Index】: R742.5
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