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《Progress in Modern Biomedicine》 2012-19
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Experimental Observation on Psychological and Behavioral Changes by Moxibustion at Different Acupoints in Model Rats of Restraint Stress

SUN Jie1,WEI Hui-fang1,ZHONG Da-kui2,WEN Jin3,ZENG Pei-jun1,LI Ying1,YANG Lei1,ZHANG Lu-fen1(1 College of Acupuncture and Massage,Beijing University of Chinese Medicine,Beijing 100029;2 Physiotherapy Department of Beijing Fangshan TCM Hospital,Beijing 102400;3 Acupuncture Department of Mentougou TCM Hospital,Beijing,102300)  
Objective: To observe the influence of moxibustion at different acupoints on behavioral changes caused by restraint stress and further discussion on regulation rule of moxibustion for psychological and behavioral changes caused by restraint stress.Meth-ods: According to the principle of stratified randomization the 35 male Wistar rats were divided into the normal group,the model group,Baihui group,Guanyuan group and Zusanli group,7 rats in each group.Except the normal group,the rest groups were tied by self-made bags for 30min each day,totally lasting 20 days to make the restraint stress model.From the 2nd day after model made,the treatment groups were treated by moxibustion for 3 cones on each acupoint,once every other day,a total of 10 times.All groups were respectively tested into the elevated plus-maze to detect the changes of the proportion of the number of entry into the elevated plus-maze open arms(OE%) and that of Residence time at the open arms(OT%) at four time points,including before and after modeling,after the 5th treatment and 10th treatment.Results: Compared with that in the groups before modeling,the OE % of the rats in Baihui group decre-ased significantly after modeling、after the 10th treatment and the OT% of the rats in Baihui group increased significantly after the 5th treatment(P0.05),after modeling the OT % of the rats in Guanyuan group decreased significantly(P0.05),after modeling the OE% and OT % of the rats,after the 10th treatment the OE % of the rats in Zusanli group all decreased significantly(P0.05,P0.05,P0.01);Compared with that in the groups after modeling,after the 5th treatment the OE% and OT% of the rats in Baihui group both increased significantly(P 0.05),after the 10th treatment the OT % of the rats in Guanyuan group increased significantly(P0.01),the OE% of the rats in Zusanli group after the 5th treatment and the OT % of the rats in Zusanli group after the 10th treatment both increased significantly(P0.01,P0.05);Compared with that in the groups after the 5th treatment,The OE % of rats in Baihui group and Zusanli group both decreased significantly after the 10th treatment(P0.05),the OE% and OT% of rats in Guanyuan group increased significantly after the 10th treatment(P0.05).Compared to that in Baihui group,the OE % and OT % of the rats in Guanyuan and Zusanli group had different changes,but no significant difference(P0.05).Conclusions: Moxibustion at different acupoints could improve anxiety and changes in emotion and behavior induced by restraint stress;Guanyuan,Baihui and Zusanli all having some anxiolytic effect,Baihui having better effect in the early period of stress,Zusanli having a reliable effect,but Guanyuan having a much more stable and obvious effect after long-term treatment,which had relative specificity.
【Fund】: 北京市自然基金资助项目(7052037)
【CateGory Index】: R245
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