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《Progress in Modern Biomedicine》 2012-24
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Effect of Coptis Root Extract on Collagen category and the Ratio of MMP-9 to TIMP-1 in Aortic Vulnerable Atherosclerotic plaque of ApoE-gene Knockout Mice

ZHOU Ming-xue1,XU Hao2,WANG Lv-ya3,LIU Wei-hong1,PAN Lin4(1 Beijing Hospital of TCM Affiliated to Capital Medical University,Beijing Institute of TCM,Beijing,100010,China; 2 Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing,100091,China; 3 Anzhen Hospital Affiliated with Capital Medical University,Institute of Cardiovascular and Pulmonary Diseases,Beijing,100029,China; 4 Institution of Clinical Medical Sciences,China-Japan Friendship Hospital,100029,China)  
Objective: To observe the effect of Coptis Root Extract(CRE) on collagen category and the ratio of MMP-9 to TIMP-1 in aortic vulnerable atherosclerotic plaque of high fat fed ApoE-gene knockout mice for exploring the possible mechanism to stabilize vulnerable plaque.Methods: Thirty-three ApoE-gene knockout mice,6-8 week age,were fed with high-fat diet for 13 weeks.After mature atherosclerotic plaque being formed,the animals were randomly allocated into three groups: the control group,the CRE group,the sim-vastatin group(as positive control),11 in each group.They were continuously fed with high-fat diet and the two drug-treated groups,respective drugs in clinically recommended dose were given for another 13 weeks.Then all mice were sacrificed by the end of experi-ment.4 sections of aortic roots in each mouse were examined by Sirius red staining.The contents of typeⅠand Ⅲ collagen and the expressions of MMP-9 and TIMP-1 in atherosclerotic plaque were detected and their ratios were caculated.Results: After treatment for 13 weeks,compared with that of the control group,the expression of typeⅠcollagen in aortic atherosclerotic plaque in CRE group was increased but not significant(P 0.05),while the expressions of type Ⅲ collagen and the ratio of type Ⅲ to typeⅠcollagen in aortic atherosclerotic plaque in CRE group and simvastatin group were significantly reduced(P0.01).Compared with that of the control group,the positive expressions of MMP-9 in plaque on CRE group and simvastatin group were significantly reduced(P0.01),the ex-pression of TIMP-1 in plaque on CRE group was significantly increased(P0.01).The expression of TIMP-1 in plaque on simvastatin group was increased but not significantly(P 0.05).The difference between the two drug treatment group was not significant(P 0.05).The ratios of MMP-9 to TIMP-1 on the two drug treatment groups were significantly reduced compared with those of the control group(P0.05,P0.01).Conclusions: In a clinically recommended dose,CRT can significantly modify the collagen category and the ratio of MMP-9 to TIMP-1 in atherosclerotic plaque of ApoE-gene knockout mice to do favor to plaque stability.
【Fund】: 国家自然科学基金项目(30973702)
【CateGory Index】: R285.5
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