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《Progress in Modern Biomedicine》 2014-10
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Effect of KLT combined with DDP on the Proliferation and Apoptosis of SiHa cell

HU Jing;MA Li-tian;YAN Ping;WEI Zhong-hai;ZHANG Ying;ZHANG Ping;TIAN Zhao-lin;ZHENG Jin;SHI Mei;WEI Li-chun;Xi jing Hospital, The Fourth Military Medical University;Department integrated traditional and western medicine of oncology, Tangdu Hospital, The Fourth Military Medical University;Student teams, The Fourth Military Medical University;65547 military hospital;Department of Traditional Chinese Medicine,Chinese,PLA General Hospital;  
Objective: To investigate the effect of KLT combined with DDP on the proliferation and apoptosis of SiHa cell. Methods: Different concentrations of KLT injection(1, 2, 4, 6, 8 mg/ml)and DDP(1.5, 3, 6, 9, 12 μg/mL)were solo applied to SiHa cells. Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. The appropriate drug concentration(KLT 6 mg/ml,DDP 3 μg/mL)were selected. Then, KLT combined with DDP injection was applied to SiHa cells. Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. Results: ① Cell proliferation were dramatically inhibited by KLT injection(25,50,100,150,200 μg/mL)and DDP(1.5, 3, 6, 9, 12 μg/mL)at 24 h,48 h(P0.05). In addition, Cell proliferation were dramatically inhibited by injected KLT combined with DDP. ②Combination therapy, apoptosis rate and inhibition rate of proliferation in SiHa cells treated by KLT combined with DDP were higher than the monotherapy. Conclusion: DDP, KLT and DDP combined with KLT can inhibit the proliferation, accelerate the apoptosis in SiHa cells. What's more, DDP combined with KLT have synergistic effect on the apoptosis in SiHa cells.
【Fund】: 国家自然科学基金面上项目(81072973);国家自然科学基金青年科学基金项目(81201749);; 省级中医药临床研究课题(lc70)
【CateGory Index】: R737.33
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