Rhythmic Beating Cardiomyocytes Derived from Human Embryonic Germ(EG) Cells In Vitro

Hua Jinlian Xu Xiaoming Dou Zhongying(Shanxi Centre of Stem Cells Engineering & Technology,Northwest Sci-Tech University of Agriculture & Forestry,Yangling 712100,China)  

Embryonic germ(EG) cells are pluripotent cells derived from primordial germ cells(PGCs) of gonads,gonadal ridges and mesenteries,analogies of fetuses,with the ability to undergo both highly self-renewal and multiple differentiation.These cells in vitro can differentiate into derivatives of all three embryonic germ layers when transferred to an in vitro environment and have the ability to form any fully differentiated cells of the body.The aim of this study is to investigate the potentiality of human EG cells differentiation into cardiomyocytes.Inducing human EG cells with the method of murine ES cells differentiation into cardiomyocytes,supplemented with 0.75%～1% DMSO,20%NBS,10~(-7)mM RA and 20% cardiomyocytes conditioned medium.Results:20 heart-like(rhythmic beating cell masses were observed in vitro culture and delayed human EG cells,which beat spontaneously from 20-120 times per minute and maintained beating for 2-15 days,periodic acid's staining(PAS),Myogoblin and α-actin immunological histology positive were all positive and reacted with K~+,Ca~(2+) and adrenalin.Relatively unorganized myofibrillar bundles or more organized sarcomeres,z-bands or a gap junction,the presence of desmosomes in a few cells of the cell masses was observed with transmision electron microscope,which initially demonstrated that these cells were cardiomyocytes.We could not get rhythmly beating cardiomyocytes with 0.75%-1%DMSO,10-7mM RA and 20% cardiomyocytes conditioned medium,but in which the percentage of cardiac α-actin immunostaining positive cells were increased.The results first demonstrated that human EG cells can differentiate into rhythmic beating cardiomyocytes in vitro and suggests that human EG cells may represent a new potent resource for cardiacomyocytes transplantation therapy for myocardium infarction.

【Key Words】： Primordial germ cells(PGCs) Embryonic germ(EG) cells Embryonic stem(ES) cells Cardiomyocytes
【Fund】： 国家自然科学基金资助项目(30200137);; 国家高技术研究与发展计划(863)项目(2002AA216161 2005AA219050);; 教育部重大科技项目(03160);; 陕西省重大科技计划;; 西北农林科技大学青年学术骨干基金资助
【CateGory Index】： R329

Download(CAJ format) Download(PDF format)

CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.