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《Progress In Biotechnology》 2003-04
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Improved Urea-LiCl Method for Extracting Total RNA from Wheat Mature Seeds

Yao Hongyan Zhao Shuangyi Xia Guangmin(School of Life Science Shandong University Jinan 250100)  
This experiment has used an improved urea-LiCl total extraction method to eliminate most of the polysaccharide in wheat mature seeds. There are three modifications compared with original method: (1) to eliminate the embryo(containing much agglutinin) of the seeds, which make the viscosity of the supernatant fluid drop down distinctly;(2) add CTAB in the lysis crude extracts to the final concentration 2‰, to eliminate most of the polysaccharide; (3)completely dissolving RNA in PEPC-treated water after RNA deposited, discard un-dissolved polysaccharide precipitate. The results showed that the purity and yield of extracted RNA of wheat mature seeds increase significantly with the improved method. ;
【CateGory Index】: S512.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 HU Qun-wen1,2,CHEN Xiao-ling1,ZHANG Zhi-e1,XIN Xia1,LU Xin-xiong1,LIU Xu1(1Key Laboratory of Crop Germplasm Resources & Biotechnology,Ministry of Agriculture / Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;2Bureau of planting management of Anqing City,Anqing 246001);A Rapid Extraction Method of High Quality Total RNA from Dry Seeds[J];Journal of Plant Genetic Resources;2010-03
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 ZHU Xiao-yu et al(College of Tobacco,Henan Agricultural University,Zhengzhou,Henan 450002);Comparison on Total RNA Extraction Methods from Tobacco Leaves[J];Journal of Anhui Agricultural Sciences;2010-15
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