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A Primary Study on Antitumor Activity of Deep-sea Microorganisms Isolated from the Tropical Pacific Ocean

WANG Xiang-min1,2 YE De-zan1,2 LIN Wen-han3 LI Ming1,2 SUN Jia1,2 HUANG Xiang-ling1,2(1 Key Laboratory of Marine Biogenetic Resources of SOA,Xiamen 361005,China)(2 Third Institute of Oceanography of SOA,Xiamen 361005,China)(3 State Key Laboratory of Natural and Biomimetic Drugs,Peking Universtiy,Beijing 100083,China)  
A total of 110 bio-products,extracted from both the supernatants by ethyl acetate and the precipitated thalli by methanol,derived from the fermented broths of 55 microorganisms including bacteria and molds isolated from deep seas in the tropical Pacific Ocean,were screened for antitumor activity by using 4 human cancer cell lines as the tested models and the techniques of methyl thiazolyl blue tetrazolium bromide(MTT)and sulforhodamine B(SRB) colorimetric assay;while the microbial strains were identified mainly by the molecular biology techniques.Some 90% of the tested 110 extracts appeared antitumor activity and 13 strains of the 55 isolates showed higher inhibitory capacities(effective concerntrations ≤16μg/ml),which may imply a possiblility in their further medical application;whereas the percentage of positive extracts was higher from the thalli than that from the supernatants and of actived strains was higher in the bacteria than that in the molds according to this study.The 50 identitied strains belong to 29 species in 21 genera while the 13 higher actived strains distribute in 8 genera.These results probably released a pilot information in the research and development of deep sea microbial resources in China.
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