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《Orthopaedil Biomechanics Maternials and Clinical Study》 2005-05
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Culture in Vitro by Multiple Enzyme Digestion and Identification of Rat Osteoblasts

Yin Meizhen, Li Shipu, Yuan Lin. Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China.  
Objective Based on the advantages and disadvantages of technique commonly for isolating osteoblasts from bone tissue, an ideal method of primary culture osteoblast was established to provide the study on bone substitutes with osteoblasts. Methods The bone tissue from the calvaria of 1 - 2day rats was digested many times to isolate and collect cells. The harvested cells were cultured in vitro. The identification was conducted by cell morphology, histochemical stain of alkaline phosphatase (ALP) and calcified nodules stain. Results The cultured cells possessed the morphological characteristic and biological behavior of osteoblasts. Conclusion This method of primary culture osteoblasts is practicable, efficient and effective, and the cultured osteoblasts can be applied to related studies in vitro.
【Fund】: 湖北省科技重点资助项目:(2002AB046)
【CateGory Index】: Q813.11
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