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ARestriction Map of Mitochondrial DNA from Ptyas mucosus Liver

Wu Ying-ji Luo Jin-xian (Department of Biology, Zhongshan Uuiversity, Guang zhou)  
The mitochondrial DNA(mtDNA)from Ptyas mucosus liver was digested with six restriction endonucleases. A unique site for Bgl Ⅰ , 2 sits for Pst Ⅰ, 3 sites for BamH Ⅰ and Bgl Ⅱ , and 4 sites for EcoR Ⅰ were found on the mtDNA, however there was no site for Sal Ⅰ. The mtDNA was cleaved by double enzymes digestions and partial digestions with the above mentioned enzymes. The framents thus obtained and their molecular weights were estimated by agarose gel electro-phoresis.A restriction map was made according to the number and the size of those fragments obtained by complete, partial and double digestions.
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