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Studying on Genetic Polymorphism of Apoliporotein B Gene 3'-End VNTR in the Han Nationality of Guangdong Province

Luo, Chao-quan Guo, Jun-ruing Yang, Ying-hao Wu, Xin-yao~* Liu, Xi-wen~1 (Department of Biochemistry, Sun Yat-Sen University of Medical Sciences * Department of Forensic Biology, Sun Yat-Sen University of Medical Sciences Guangzhou, 510089)  
The bloodstain DNA was extracted from 100 unrelated individuals of Han Nationality of Guangdong Province and their patterns of apoB gene 3'-epd VNTR were typed by PCR. The results showed that nineteen alleles were detected and their sizes range from 510bp to 1200bp. The allele frequencies were 0.005—0.195. the heterozygosity of this locus was 88.80% and the paternity exclusion probability was 0.6395. X~2 test demonstrated that allele frequency distribution was consistent with Hardy-Weinberg equillibrium. The number of spoB VNTR allele and allele frequency of Han nationality were different from those of American Caucasian population.The results of genotyping of family members were in good agreement with Mendelian Law. So these methods would be valuable for Medical Goneties, Anthropology and Foronsic ciences.
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