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Cloning and Homologous Analysis of Sporamin A Gene Coding Region Sequence of Ipomoea batatas

CHEN Ke gui,WANG Hai yan,ZHANG Yi zheng (College of Life Sciences,Sichuan University,Chengdu 610064)  
The coding region of sporamin A gene from sweet potato Nanshu 88,a widely cultivated variety in China,was amplified by the polymerase chain reaction (PCR) with primers designed on the sequence of sporamin gene gSPO Al.The amplified fragment was cloned and sequenced.This coding region was 657 bp in size and encoded a peptide of 219 amino acid residues,in which there was a signal peptide with 37 amino acids and a mature peptide of 182 amino acids.The molecular weight of the mature sporamin was 20 kD.Comparison of this sequence with those of other six sporamin A genes in GenBank showed that the nucleotide homology was as high as 90%,indicating that the sequence of sporamin coding region was very conserved.Although the total variety among all seven sporamin genes was about 10% the variety between each two sporamin genes was less than 7%.
【Fund】: 四川省“九五”重点科学技术研究资助项目
【CateGory Index】: Q503;Q78
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