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《Chinese Journal of Biochemistry and Molecular Biology》 2011-04
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Exogenetic Overexpression of ST3Gal Ⅰ Increases the Ability of Adhesion and Invasion to ECM in Breast Cancer MCF-7 Cells

CUI Hong-Xia1),2),YUE Li-Ling1),LIU Ji-Cheng1)*(1) Department of Pharmacology,Institute of Medicine,Qiqihar Medical College,Qiqihar 161006,China;2) Heilongjiang University of Chinese Medicine,Harbin 150040,China)  
To study the effect of exogenetic overexpression of α 2,3 sialyltransferase(ST3Gal Ⅰ) on the abilities of adhesion and invasion in MCF-7 cells,we constructed pEGFP-N1-ST3Gal Ⅰ eukaryotic expression vector.The recombinant vector was transfected into MCF-7 cells by GenEscortTM Ⅱ.MCF-7 cells were divided into 3 groups: mock cells(M),parental cells(P) and ST3Gal Ⅰ-overexpression(ST3).The expression of fusion protein ST3Gal Ⅰ-EGFP in cells was observed using fluorescence microscope.The expression levels of ST3Gal Ⅰ mRNA and protein were examined by RT-PCR and Western blotting.The amount of α 2,3-sialic acids on the cell surface,which is the downstream product of ST3Gal Ⅰ,was detected by flow cytometry.The ability of adhesion and invasion of MCF-7 cells to Matrigel was analyzed by using adhesion assay and transwell assay.The results suggested that fluorescence was observed in whole cell in P,whereas that is in the cytoplasm in ST3.The exogenetic expression levels of ST3Gal Ⅰ mRNA,protein and the amount of α 2,3-sialic acids on cell surface in ST3 group were significantly increased compared with that in the M group and P group(P 0.05).The abilities of adhesion and invasion of the cells in ST3 group were markedly higher than those in the other 2 groups(P 0.05).The technology of transfection can effectively enhance the exogenetic expression of ST3Gal Ⅰ and increase cell adhesion,migration and invasion to Matrigel in MCF-7 cells and can promote tumor metastasis.This may be meaningful for seeking novel target for therapeutic approaches in breast cancer.
【Fund】: 国家自然科学基金(No.30772751)项目资助~~
【CateGory Index】: R737.9
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