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《Chinese Journal of Biochemistry and Molecular Biology》 2013-04
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Overexpression of Gastrin Promotes the Proliferation,Migration and Invasion of Gastric Cancer Cells

LIU Jun1),ZHOU Jian-Jiang1),2) ,XIE Yuan1),ZHAO Yan1),ZENG Yi1)(1) Key Laboratory of Molecular Biology of Guiyang Medical College,Guiyang 550004,China; 2) Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)  
The increased gene expression of gastrin by 42 folds in primary gastric cancer patients at the metastatic lymph node suggested its close linkage to the metastasis of gastric cancers.We constructed a eukaryotic expression vector pcDNA3.1-gastrin and transfected it into the gastric cancer cells AGS and SGC-7901 and establish the stable gastrin-overexpression cell lines.The proliferation,migration,invasion of transfected cells were detected by MMT,cell wound healing,and Transwell assay;and the matrix metalloproteinase-2(MMP-2) protein level in the medium was detected by ELISA.The results showed that the relative proliferation rate and migration distance of wound edge in gastrin-overexpressed cells were higher than those of empty vector in transfected control cells;as well as the cell numbers that penetrated to the lower chambers of Transwells and the medium levels of MMP-2 secretion.Our findings suggested that gastrin might increase the migration and invasion of gastric cancer cells with the incrase of MMP-2 secretion,which was required for gastric cancer metastasis.
【Fund】: 国家自然科学基金(No.31060122);; 贵州省科技攻关计划项目(No.黔科合SY字[2011]3067);; 贵州省科学技术基金(No.黔科合J字[2012]2039)~~
【CateGory Index】: R735.2
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 CUI Hong-Xia1),2),YUE Li-Ling1),LIU Ji-Cheng1)*(1) Department of Pharmacology,Institute of Medicine,Qiqihar Medical College,Qiqihar 161006,China;2) Heilongjiang University of Chinese Medicine,Harbin 150040,China);Exogenetic Overexpression of ST3Gal Ⅰ Increases the Ability of Adhesion and Invasion to ECM in Breast Cancer MCF-7 Cells[J];Chinese Journal of Biochemistry and Molecular Biology;2011-04
2 SHAN Chang-Liang,ZHANG Shuai,ZHANG Xiao-Dong,YE Li-Hong(Key Laboratory of Bioactive Material of Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China);Hepatitis B Virus X Protein Mutant (HBxΔ127) Promotes Hepatoma Cells Migration via ERK-induced Upregulation of Calpain Small Subunit 1[J];Chinese Journal of Biochemistry and Molecular Biology;2011-05
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Wang Mingyu;Wang Xuhong;Tian Chunduo;et al(Shandong Cancer Hospital,and Institute,Jinan 250117);The Serum Level of Gastrin in Patients with Benign Alimentary Canal Diseasesand Gastric Cancer[J];JOURNAL OF QILU ONCOLOGY;1996-01
2 Ping'an ZHU~(1,2*) Deming TAN~1 Zhongtian PENG~1 Fei LIU~1 Lin SONG 1 Department of Infectious Diseases,Xiangya Hospital,Central South University,Changsha 410008,China; 2 The Seventh Hospital of Shenzhen,Shenzhen 518081,China;Polymorphism Analyses of Hepatitis B Virus X Gene in Hepatocellular Carcinoma Patients from Southern China[J];生物化学与生物物理学报(英文版);2007-04
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