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《Chinese Journal of Biochemistry and Molecular Biology》 2015-10
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Antibacterial Mechanism of Ethanolic Extract of Hibiscus sabdariffa Against Escherichia coli and Staphylococcus aureus

LI Meng-Miao;XIE Kun-Peng;SUI Jia-Qi;XIE Ming-Jie;School of Life Sciences,Liaoning Normal University,Key Laboratory of Biotechnology and Drug Discovery of Liaoning Province;  
Hibiscus sabdariffa( HS) has extensive physiological activities including anti-oxidant,antitumor and anti-bacterial activities. However,the anti-bacterial mechanisms were rarely reported. Here we investigated the effect of the ethanol extract HS on cell membrane integrity,protein and nucleic acid synthesis in Escherichia coli and Staphyloccocus aureus. We systematically investigated the anti-bacterial mechanism of the ethanol extract HS. Conductivity and macromolecular leakage results showed that the ethanol extract HS only slightly damaged the membrane of bacteria,which suggested that the antibacterial activity did not relate to the cell membrane. SDS-PAGE and DAPI staining results showed that the ethanol extract HS could potently inhibit the synthesis of proteins and nucleic acids. Agarose gel electrophoresis and the UV-visible spectrophotometric results indicated that the ethanol extract HS could integrate with DNA: at low concentration ratio of DNA and HS,the ethanol extract HS could insert into DNA double helix; while at high concentration ratio,the ethanol extract HS interacted with DNA through hydrogen bond. Our results indicated that the anti-bacterial mechanism of the ethanol extract HS was mainly through combining with DNA,which inhibited the transcription and the translation of DNA,andthus inhibited the biological function of bacteria.
【Fund】: 辽宁省教育厅科学研究一般项目(No.L2013412);; 大连市科技计划项目(No.2013E13SF108)~~
【CateGory Index】: R285
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