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Stabilization of Iron-containing Superoxide Dismutase via Hydrophilization

Liang, Yong-tao;Liu, Wei-hua;Sun, Qi-an;Luo,Gui-min;Yang,Tong-shu(The National Laboratory of Enzyme Engineering, Jilin University, Changchun 130023)  
Reductive alkylation of amino groups in iron-containing superoxide dismutase with glyoxylic acid in the presence of sodium cyanoborohydride results in great stabilization of the enzyme against irreversible thermoinactivation. At 80℃ the half life of modified enzyme is about 2times longer than that of native one.
【Fund】: 国家自然科学基金
【CateGory Index】: Q550.3
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