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Purification of Recombinant Human Tumor Necrosis Factor-α Expressed in E.coli

Long, Na; Tang,Zhi-Hua;Yu, Guang-Hua; Shi, Feng-XiaZhu, You-Hua; Ding, Shu-Biao; Xu, Xiang-Yu(The Millitary Medical lnstitute of Nanjing Command, PLA, Hanjing 210002 )  
Recombinant human tumor necrosis factor-α(rhTNF-α) expressed in E. coli was isolated and purified by ion exchange chromatography.Through step wash elution, the chromatographic peak containing rhTNF-αwas picked up.Specific activity of TNF-αwas 2.4×108U/mg.The recovery rate was 77.2%. This study constructed the basis for large scale preparation and clinical use of rhTNF-α.
【CateGory Index】: R392-33
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