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The Effect of 5-Aza-2'-deoxycytidine (5-Aza-CdR)on Cell Differentiation and DNA Methylase of HL-60 Cells

He,Zhong-Xiao;Gu, Yu;Rao, Hong;Bai,Jian-Shi; Ma, Qing (Department of Biology, Beijing Normal University, Beijing 100875)  
The differentiation index of HL-60 cells was measured after being treated with 5-aza-CdR by using The NBT staining method. The value of differentiation index is 15% or so when the concentration of 5-aza-CdR is 0. 5μmol/L. The DNA methylase activities of HL-60 cells treated with the drug were assayed by using 3H-methyl-S-adenosylmethionine (3H-SAM) as methyl donor. The DNA methylase activities of HL-60 cells were obviously decreased by the treatment of 5-aza-CdR. This change was related to the concentration of 5-aza-CdR. The DNA methylase activities of different HL-60 cells were also compared by using different DNA substrates in vitro. All these results showed that the 5-aza-CdR could induce the differentiation of HL-60 cells and it was closely related to the activity of DNA methylase of HL-60 cells.
【Fund】: 国家自然科学基金
【CateGory Index】: Q26
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【Co-references】
Chinese Journal Full-text Database 2 Hits
1 DONG Yu_wei,HOU Jin_hui,ZHU Bi_cai,PANG Yong_hong(School of life Science,Xuzhou Normal University,Xuzhou,221116,Jiangsu,China);Concepts related to epigenetics and their advances[J];Journal of Biology;2005-01
2 LIU Lian-ye1 ,DUAN Xiao-ming2, HU Yi-kui1,GUI Ruo-hu2,ZHENG Xin-ping2,HE Xiu-sheng1,CAO-Jian-guo1 1Nanhua university,Hengyang 421001,China,2Hengyang City Central Hospital, Hengyang 421001,China;Methylation of the 5' CpG island of the FHIT gene and the relationship to it's inactivation[J];Journal of Modern Oncology;2006-10
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