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Ce ̄(3+) Binding to G-Actin and Consequent Conformation Change and Polymerization

Sun Hong-Ye; Liu Qun; Yao Guang-Qing; Wang Kui (Inorganic Chemistry Department,School of Pharmaceutical Sciences Beijing Medical University, Beijing 100083)  
Ce ̄(3+) binding to G-actin and the consequent micro-conformation change were studied by fluorescence probe of aedans attached to G-actin. Ce ̄(3+) competes the high affinitive divalent binding site with Ca ̄(2+) at low concentration (Ce  ̄(3+)/actin molar ratio 1 ). The fluorescence enhancement of aedans resulting from Ce ̄(3+) substituting Ca ̄(2+) was similar to that resulting from Mg ̄(2+) replacing Ca ̄(2+). While the fluorescence was quenched when Ce ̄(3+)/actin molar ratio is larger than 1, which indicated that the micro-conformation of Cys374 has two different state at low and high Ce ̄(3+) concentration. The life time measurement of aedans by time resolved fluorescence technique also agree with the above results. The secondary structure characterized by circular dichroism showed that actin increased the secondary structure at low molar ratio of Ce ̄(3+) /actin(M O. 4) and lost it at high molar ratio. The polymerization of Ce ̄(3+) -actin complexes in absence of free ATP showed that the polymerization was enhanced at low Ce ̄(3+) concentration and inhibited at high concentration. In the presence of free ATP, the polymerization was enhanced in the range of Ce ̄(3+) /actin studied.
【Fund】: 北京大学稀土材料化学 应用研究开放实验室 自然科学基金
【CateGory Index】: Q51
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【References】
Chinese Journal Full-text Database 2 Hits
1 YANG Wei dong 1,WANG Ting 3,LEI Heng yi 2,LIU Jie sheng 1,YANG Yan sheng 2 (1.College of Life Sciences and Biotechnology,Jinan University,Guangzhou 510632; 2.College of Chemistry and Chemical Engineering,Zhongshan University, Guangzhou 510275;Progress in Studies on Biological Effects of Rare Earth[J];CHINESE RARE EARTHS;2000-03
2 QIU Guan ming 1,LI You rong 2,CHEN Shi yan 3,ZHOU Lan xiang 2,LI Wei 3,ZHANG Ming 2 (1.Changchun College of Optics & Fine Mechanics,Changchun 130022,China; 2.College of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou 225002,China; 3.Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China);Advance in Research on Dose-dependent Mechanism of Rare Earths on Organism[J];Chinese Rare Earths;2003-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Jianfei; Chen Jinqui; Xin Swzhi ;Duan Lizhen(Anhui Agrotechnical Teachers College, Fengyang,233100)Gan Wuer ;He you zhao(Vniversity of Science and Technology of china, Hefei,230026);Effects of Rare Earth Micro Fertiliazer on Chlorophy 11 Content of Leares and Yield of Tomato[J];;1997-02
2 WANG Ling1, HONG Fa-shui2(1.Library, Suzhou Vniversity, Suzhou 215006, China;2.Colegge of Life Science, Suzhou University, Suzhou 215006, China);Progress of Study on Rare Earth Elements on Chlorophyll Formation[J];Journal of Anhui Technical Teachers College;2004-01
3 Wei Gang He Xiaochao(Experimantal Center for Forest Biology) (Department of soil and water conservation);Studies on Application Technique of Rare Earth in the Young Forest Stand of Black Locust[J];Journal of Beijing Forestry University;1989-04
4 Wei Gang Zha Duolu (Dept.of Science Research,BFU) (Dept. of Water and Soil Conservatioin,BFU) Dong Yuan Wu Jingke (Foest Resource College,BFU) (Dept.of Science Research,BFU);Studies on the Beneficial Results of Rare Earths and Composite Liquid Fertilizers in Increasing the Yield of Apple Trees in Sandy Soil[J];Journal of Beijing Forestry University;1991-01
5 Zhang Yuanbing,Liu Airong,Zhang Xueping,Du Xiaoming(School of Life Science,College of Science and Technology of Anhui.Anhui Fengyang:233100);Impact of hormones and rare earth on the seedling growth of Paeonia suffruticoca[J];Quarterly of Forest By-product and Speciality In China;2005-06
6 MA Jian-jun,ZHU Jing-tao,CHEN Fang,WU He-ping,ZOU De-wen (Hebei Normal University of Science and Technology, Changli 066600,China);Alleviation effects on unitary and mixed rare-earth elements on the accumulation of heavymetals in rapes[J];Journal of Agricultural University of Hebei;2004-01
7 Tang Yuxia;Meng Chunxiang;Li Chunbo(Soil and Fertilizer Institute,Hebei Acadermy of Agricultural and Forestry Sciences,Shi Jiazhuang 050051);The Content and Fertilizer Effciency of Rare Earth in Phosphate Fetilizer from Different Place of Production[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1994-S1
8 Xu Xinyu Zhang Yumei (Soils and Fertilizers Institute CAAS,Beijing,100081)Lu Zhongyi Liu Yuzhen Chen Mingshun (Xinjiang Grassland Managing General Station,Urumchi,830049);EFFECT OF RARE EARTH APPLIED TO FORAGE PRODUCTION[J];Pratacultural Science;1993-06
9 WANG Li-ke~1, HONG Fa-shui~2(1.Animal Science Department, Anhui Technical Teachers College, Fengyang 233100, China; 2.College of Life Sciences, Suzhou University, Suzhou 215006, China);Effect of eurium chloride on the vigor of aged alfalfa seeds[J];Acta Pratacultural Science;2003-06
10 JI Hongbing 1,2 ,WANG Lijun 2 ,DONG Yunshe 2 ,WANG Shijie 2 ,LUO Jianmei 1 ,SUN Yuanyuan 1 (1.Key Laboratory of Resource,Environment and GIS,Department of Geography,Captial Normal University,Beijing100037;2.Institute of Geographical Sciences and Natural Resources Research,Beijing100101;3.The State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Guiyang550002);An Overview On The Study Of Biogeochemical Cycle For Rare Earth Elements(REEs)[J];Progress In Geography;2004-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yang Yuangen; Liu Congqiang; Yuan Keneng; He Zhenli (Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002; College of Environment and Assources, Zhejiaing University , Hangzhou 310029);LATERITE FORMATION PROCESS IN SOUTHERN CHINA AND ITS RARE EARTH ELEMENT (REE) GEOCHEMISTRY[J];QUATERNARY SCIENCES;2000-05
2 Ma Guangzhi(Department of Biology, South China Normal University, Guangzhou 510631)Lin Haoran Zhang Weimin(Department of Biology, Zhongshan University, Guangzhou 510275);EFFECT OF Cd~(2+) ON GONADOTROPIN SECRETION OFPITUITARY FROMCOMMON CARP (Cyprinus carpio L.) IN VITRO[J];Zoological Research;1995-03
3 WANG Xin-ying,JI Ming,LI Zhi-guo,LU Xiao-jie,CHENG Jiong-jia,BI Shu-ping~*(State Key Laboratory of Coordination Chemistry& Key Laboratory of Analytical Chemistry for Life Science of MOE,Department of Chemistry,Nanjing University,Nanjing 210093);Progress of Research on the Interactions Between DNA and Metal Ions[J];Journal of Analytical Science;2005-05
4 FENG Zhi-Xiang, ZHANG Shu-Gong , LIU Qi-MinYANG Kui-Yue, NI Jia-Zuan(Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Chanchun, 130022)MIAO Jian, YOU Shu-Heng (Department of Biochemistry, Henan Medical University, Zhengzhou);Interactions of Rare Earth Ions with Bovine Blood Cu(Zn)-Superoxide Dismutase[J];Chemical Research In Chinese Universities;1995-07
5 LIU De Long 1,* , SUN Da Ye 2, YANG Yan Sheng 3 [WT5”BX](1. Department of Chemistry, 2. Department of Biology, Hebei Normal University, Shijiazhuang 050091, China; 3. Department of Chemistry, Zhongshan University, Guangzhou 510275, China) [WT;Studies on Plant Extracellular Calmodulin by Lanthanide Luminescence Probe[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;2000-06
6 HUO Hong 1 , HU Xiang 2, GUAN Hong Wei 2 WANG Xing Fu 1, CHE Xun 1, HUANG Wei Dong 1 (1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China; 2. No.1 Subsidiary Hospital, Dalian Medical University, Dali;Studies on Human Breast Cancer Tissue With Infrared Spectrometry[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;2000-08
7 HUANG Zhong-Xian;ZHENG Qi;GAO Hong-Ying;GU Wei-Qiang;LIU Fang(Department of Chemistry,Fudan University,Shanghai,200433);The Reactions Between Lanthanons and Metallothionein[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-02
8 YANG Bin-Sheng(Institute of Molecular Science, Shanxi University, Taiyuan, 030006)T. G. St.Pierre, J. Webb(Murdoch University, Perth WA6150, Australia);The Effect of Europium (Ⅲ) on Iron Uptake by Apoferritin[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-05
9 HUO Hong,WANG Xing-fu,CHE Xun,and HUANG Wei-dong Dalian Institute of Chemical Physics, Chinese Academy of Sciences,Dalian 116012,China;Study on the Characters of Hydrogen Bonds in Protein and Nucleic Acid of the Breast Cancer Tissues[J];Spectroscopy and Spectral Analysis;2001-05
10 ZHANG Li_xia,LIANG Li_fang,CHEN Chao_qiu (Department of Chemistry,Guangxi Teachers College,Nanning 530001,China);Mitigative Effect on Cd Pountion through Nd(Ⅲ) and Its Complex Nd(POA)_2.hq[J];Journal of Guangxi Teachers College;2001-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XU Xiang-gui et al (Department of Biology,Suzhou Science and Technology College,Suzhou,Jiangsu 215009);Effect of Lanthanum on Activity of Isolated Rat's Ileum[J];Journal of Anhui Agricultural Sciences;2006-12
2 HU Jian, JIA Yu, LIU Xiang Tao △, LI Rong Chang, WANG Kui (Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, Beijing 100083,China);Effects of lanthanum on alkaline phosphatase[J];Journal of Beijing Medical University;2003-02
3 JI Hongbing 1,2 ,WANG Lijun 2 ,DONG Yunshe 2 ,WANG Shijie 2 ,LUO Jianmei 1 ,SUN Yuanyuan 1 (1.Key Laboratory of Resource,Environment and GIS,Department of Geography,Captial Normal University,Beijing100037;2.Institute of Geographical Sciences and Natural Resources Research,Beijing100101;3.The State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Guiyang550002);An Overview On The Study Of Biogeochemical Cycle For Rare Earth Elements(REEs)[J];Progress In Geography;2004-01
4 LIN Jun~1,LI Tao~(1,2),SHEN Hong~(2),ZHOU Pei-jiang~(1), SONG Li-rong~(3), SHEN Yin-wu~(3), LIU Yong-ding~(3)(1.Department of Environmental Science, Wuhan University, Wuhan 430072;2. Environmental Monitoring Centerof Hubei Province, Wuhan 430072;3. Institute of Hydro-biology, Chinese Academyof Sciences, Wuhan 430072);A Kinetic Study on Effect of Cerium on Microcystis Growth and Enrichment of Cerium in Microcystis[J];Environmental Science and Technology;2004-01
5 WANG Li-xin, FAN Wei-guo(Research Institute for Fruit Resources of Karst Mountain in Region, Guizhou University, Guiyang Guizhou 550025, China);Photosynthetic Characteristics of Citrus ichangensis[J];Guizhou Agricultural Sciences;2009-02
6 LI Wei 1,LIANG Jiansheng 1, PAN Zhiming 1,LI Yourong 1,QIU Guanming 2 (1 College of bioscience and biotechnology, Yangzhou Univ., Yangzhou, 225009,China; 2 Changchun College of Optics & Fine Mechanics, Changchun, 130022,China);Studies on biological dosage effect of adjustment of rare earth element in CaM level[J];Journal of Guangxi Agricultured and Biological Science;2002-01
7 GONG Ju-fang *,ZHANG Zi-gui *,SHEN Xiu-ying *, XU Xiao-lu,CAI Rong-gang * ( * College of Chemistry & Life Sciences,Zhejiang Normal University,Jinhua 321004 China);Effects of Cerium on Proportion of Internal Organs of Rats and Anti-oxidation Ability in Liver and Kidney[J];Industrial Health and Occupational Diseases;2005-02
8 LI De-yan1,2,PAN Xue-jun2(1.Department of Physics,Anshun College,Anshun,Guizhou 561000,China;2.Research Institute for Fruit Resources of Karst Mountain Region of Guizhou University,Guiyang,Guizhou 550025,China);Comparison of the Photosynthetic Characteristics of Wild V.Quinquangularis Rehd in Guizhou[J];Northern Horticulture;2009-11
9 Wang Shaoxian Peng Keqin(Key Laborary of Plant hormones, Hunan Agricultural University,Changsha 410128);Research Advance of Rare Earth Application on Agriculture and Mechanism[J];Journal of Hebei Agricultural Sciences;2002-01
10 Wang Shaoxian Peng Keqin Xiao Langtao Xia Shitou(College of Science and Technology, Hunan Agriculture University, Changsha 410128);Research Advances on Crop Nutrition and Yield in Creasing Mechanism[J];Journal of Hebei Agricultural Sciences;2002-03
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