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Ce ̄(3+) Binding to G-Actin and Consequent Conformation Change and Polymerization

Sun Hong-Ye; Liu Qun; Yao Guang-Qing; Wang Kui (Inorganic Chemistry Department,School of Pharmaceutical Sciences Beijing Medical University, Beijing 100083)  
Ce ̄(3+) binding to G-actin and the consequent micro-conformation change were studied by fluorescence probe of aedans attached to G-actin. Ce ̄(3+) competes the high affinitive divalent binding site with Ca ̄(2+) at low concentration (Ce  ̄(3+)/actin molar ratio 1 ). The fluorescence enhancement of aedans resulting from Ce ̄(3+) substituting Ca ̄(2+) was similar to that resulting from Mg ̄(2+) replacing Ca ̄(2+). While the fluorescence was quenched when Ce ̄(3+)/actin molar ratio is larger than 1, which indicated that the micro-conformation of Cys374 has two different state at low and high Ce ̄(3+) concentration. The life time measurement of aedans by time resolved fluorescence technique also agree with the above results. The secondary structure characterized by circular dichroism showed that actin increased the secondary structure at low molar ratio of Ce ̄(3+) /actin(M O. 4) and lost it at high molar ratio. The polymerization of Ce ̄(3+) -actin complexes in absence of free ATP showed that the polymerization was enhanced at low Ce ̄(3+) concentration and inhibited at high concentration. In the presence of free ATP, the polymerization was enhanced in the range of Ce ̄(3+) /actin studied.
【Fund】: 北京大学稀土材料化学 应用研究开放实验室 自然科学基金
【CateGory Index】: Q51
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