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《Chinese Journal of Bioprocess Engineering》 2005-03
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Preparation of (S)-mandelic acid and its derivatives by enantioselective degradation of racemic mandelates with Pseudomonas putida CGMCC1388

HUANG Han-rong,PAN Jiang,XU Yi,XU Jian-he(Laboratory of Biocatalysis and Bioprocessing,State Key Laboratory of Bioreactor Engineering,East China Universityof Science and Technology,Shanghai 200237,China)  
A mandelate-degrading bacterium,Pseudomonas putida CGMCC1388,was newly isolated from soil using benzoylformic acid as sole carbon source.It could degrade(R)-mandelic acid with high enantioselectivity(E=130).Supplement of mandelic acid,benzoylformic acid or benzoic acid to the culture medium as the enzyme inducer significantly enhanced the degradation activity of the bacterial cells.The optimal concentrates of these inducers were 4 g/L,4 g/L and 2 g/L respectively.The optimal temperature and pH for the mandelate degradation were 30 ℃ and 6.0 respectively,and the optimal substrate concentration was 60 mmol/L.The apparent kinetic constants K_(m) determined was 47 mmol/L.Using the resting cells as biocatalyst,(S)-mandelic acid,(S)-p-hydroxymandelic acid and(S)-p-chloromandelic acid were readily prepared,with moderate to high yields and excellent enantiopurity (99%e.e.).
【Fund】: 国家自然科学基金重大项目(203900506);; 国家973计划(2003CB716008)
【CateGory Index】: TQ922.9;
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