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《Chinese Journal of Bioprocess Engineering》 2007-03
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Extraction and isolation of Lentinan from of lentinus edodes

MIAO Jian,YANG Wen-ge,ZHOU Bin,LU Xiu-tao(College of Life Science and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China)  
The crude Lentinan was firstly extracted by water-extracting and alcohol-depositing,and was further deproteinzated by papain and Sevage method.The purification of the polysaccharides was carried out by DEAE-cellulose chromatography and Sephadex G-200 column chromatography.Two fractions were obtained,named as Len1-A,Len2-A.The two fractions were examined by optical rotation,Sepharose 4B column chromatography and IR,the results showed that two fractions were a homogenous polysaccharide,Len1-A,Len2-A are α-pyranoses.The total sugar content of Len1,Len2 were 91.5 % and 92.3 %.The optical rotation were +5.6 ° and +11.2 °.Their MW were 375×105and 718×105.
【CateGory Index】: R284
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