Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Bioprocess Engineering》 2007-03
Add to Favorite Get Latest Update

Extraction and isolation of Lentinan from of lentinus edodes

MIAO Jian,YANG Wen-ge,ZHOU Bin,LU Xiu-tao(College of Life Science and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China)  
The crude Lentinan was firstly extracted by water-extracting and alcohol-depositing,and was further deproteinzated by papain and Sevage method.The purification of the polysaccharides was carried out by DEAE-cellulose chromatography and Sephadex G-200 column chromatography.Two fractions were obtained,named as Len1-A,Len2-A.The two fractions were examined by optical rotation,Sepharose 4B column chromatography and IR,the results showed that two fractions were a homogenous polysaccharide,Len1-A,Len2-A are α-pyranoses.The total sugar content of Len1,Len2 were 91.5 % and 92.3 %.The optical rotation were +5.6 ° and +11.2 °.Their MW were 375×105and 718×105.
【CateGory Index】: R284
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 Han Xue Liu Anjun et al.;Comparation on preparation and biological function of polysaccharde from different sources[J];China Feed;2008-07
2 ZHANG Kai-zheng,YANG Yue-huan,LI Guang-hui,LI Jie-jun,ZHANG Ze-yan,LI Gang (School of Biotechnology Engineering,Sichuan University of Science & Engineering,Zigong 643000,China);Research Advances on the Extracting Methods of Lentinan[J];Journal of Sichuan University of Science & Engineering(Natural Science Edition);2009-06
3 LI Lei,WANG Wei-guo(School of Biotechnology Engineering,Henan University of Technology,Zhengzhou 450052,China);RESEARCH PROGRESS OF FUNGAL POLYSACCHARIDES ON THE PHARMACOLOGICAL ACTIONS AND ITS EXTRACTION AND PURIFICATION[J];Journal of Henan University of Technology(Natural Science Edition);2008-02
China Proceedings of conference Full-text Database 1 Hits
1 Liya Ma Peisheng Yan (School of the Marine,Harbin Institute of Technology at Weihai No.2,West Culture Road,Weihai,Shandong Province 264209);EXTRACTION OF INTRA-POLYSACCHARIDES FROM FUNGAL MYCELIUM BY ENZYMATIC DIGESTION[A];[C];2009
Chinese Journal Full-text Database 7 Hits
1 Luo Yi Li Dong (Analysis Department, Beijing Research Institute for Nutritional Resources,Beijing,100054);Research Advances in Lentinan[J];FOOD AND FERMENTATION INDUSTRIES;2000-04
2 ;黄桃水溶性多糖的化学结构分析[J];Science and Technology of Food Industry;2005-06
3 TIAN Guang-hui ( Department of Chemistry,Shaanxi Institute of Science and Engineering,Hanzhong 723000,China );Improvement on the extractive technique of polysaccharide of lentinus edodes[J];Journal of Yanan University(Natural Science Edition);2002-02
4 MA Chang-qing1, XIA Rong1, PENG Yan1, HE Xiu-ping2(1.Department of Pharmaceutical Analysis; 2.Undergraduate Trainee of the Year 1997, College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);A Study of Methods for Extraction of Lentinan and Amino Acids From the Foot Body of Lentinus Edodes[J];Herald of Medicine;2003-06
5 CHEN Qun 2,YANG Gui wen 1,An Li guo 1* (1.The Provincial Key Laboratery,Department of Biology,Shandong Normal University,Jinan 250014,China;2.Department of Biology & Chemistry,Huainan Normal College,Huainan 232001,China);Isolation,purification and analysis of a polysaccharide from Ginkgo biloba seed[J];Chinese Pharmaceutical Journal;2002-05
6 WANG Wei-guo,WU Qiang,HU Bao-kun,BAO Dong-wu,ZHAO Yong-liang 1.Biochemical Engineering Dept.,Nanyang Institute of Technology,Nanyang,Henan 473004,PRC.2.Medical School,Nanyang Institute of Technology,Nanyang,Henan 473002,PRC.3.Agricultural Dept.,Sichuan Agricultural University.Ya'an,Sichuan,625014,PRC.;Comparative Study on Determination Methods of Residual Protained in Grifolan[J];Edible Fungi of China;2003-01
7 Sun Peilong;Jiang Tongjun(Zhejiang University of Technology, Hangzhou 310014);A Review of Eritadenine[J];EDIBLE FUNGI OF CHINA;1995-02
Chinese Journal Full-text Database 10 Hits
1 LI Jian,LI Feng,WANG Hou-wei(Chinese Materia Medica Faculty,Shandong University of Traditional Chinese Medicine,Jinan 250355,China);Related study on properties and protein content of traditional Chinese drugs[J];Journal of Shandong University of Traditional Chinese Medicine;2009-03
2 ZHAO Da-yun,YANG Li-e,ZHAO Yi-hua,HU Xue-hua( School of Agriculture &Biology,Shanghai Jiaotong University,Shanghai 2011 01,China);The Comparison of Extraction and Determination Methods Applied for Alfalfa Polysaccharides[J];Journal of Shanghai Jiaotong University (Agricultural Science);2004-03
3 FENG You1,2,WANGFeng-shan1,2,ZHANGTian-min1,TAN Hai-ning1,2(1.National Glycoengineering Research Center,2.School ofPharmacetical Sciences,Shandong University,Jinan250012,China);Research advances of polysaccharide drugs[J];Chinese Journal of Biochemical Pharmaceutics;2008-02
4 Huang Aixia Zou Xiaoting;Review on the immune modulating effect of lentinan and its application in poultry[J];China Feed;2007-10
5 Zhu Xiaomei Tian Guanghui (Department of Chemistry,Shanxi University of Technology,Hanzhong, 723000);Optimizing Extraction Process of Polysaccharide from Root of Lentinus Edodes[J];Food and Fermentation Industries;2004-03
6 Wang Qin~(1,2) Zou Jie~1 Wen Qibiao~11(College of Light Industry and Food,Zhongkai Agrotechnical College,Guangzhou 510225,China)2(College of Light Industry and Food,South China University of Technology,Guangzhou 510640,China);Extraction of Polysaccharide from Ginkgo Biloba Seed by Ultrasonic Wave[J];Food and Fermentation Industries;2006-01
7 Zhang Nan~(1,2) Wu Yuangen~(1,3) Mo Liping~4 Qiu Shuyi~(1,3) Wang Wenping~(1,3) 1(Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy,Guizhou University,Guiyang,550003,China) 2(College of Life Science,Guizhou University,Guizhou 550025,China) 3(College of Chemical Engineering,Guizhou University,Guizhou 550003,China) 4(Guiyang Productivity Promotion Center,Guizhou 550002,China);Study on the Optimal Phosphorylation of Lentinan[J];Food and Fermentation Industries;2007-12
8 WANG Bin~ 1,3 LIAN Bin~ 1,2 (1.The Provincial Key Laboratory of Fermentation Engineering and Bio-Pharmaceutical,Guizhou University,Guiyang,Guizhou 550003,China;2.Key Laboratory for the Exploitation of Southwestern Resources & the Environmental Disaster Control Engineering,Ministry of Education,Chonqing 400044,China;3.Henan Science and Technology College,Xinxiang,Henan 453003,China);Progress on polysaccharides from edible and medicinal fungi[J];Food and Machinery;2005-06
9 CHENG Chao (School of Biological Science and Technology,Hubei Institute for Nationalities,Enshi,Hubei 445000,China);Extraction technology optilization of polysaccharide of Colored-Mushroom[J];Food & Machinery;2006-01
10 ZHU Yin-ling1,2,TAN Zhu-jun2,LI Wei-cai3,LIU You1(1. Modern Biochemistry Experimental Center,Guangdong Ocean University,Zhanjiang 524088,China;2. Faculty of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China;3. South Subtropical Crops Institute,Zhanjiang 524091,China);Study on extraction of Averrhoa caramhola L. polysaccharides[J];Science and Technology of Food Industry;2008-03
China Proceedings of conference Full-text Database 2 Hits
1 HE Zhi-wei1 LIU Zhong-dong1 SHAO Bin2 WEI Guo-hua1 XU Xin-de2(1.School of Oil and Food Henan University of Technology, Zhengzhou 450052,China;2.Zhejiang Xinchang Pharmaceutical Factory,Xinchang 312500,China);Study on the removed protein of vaccinium bractum thunb leave extract[A];[C];2007
2 Ji Yu-bin, Guo Shou-dong, Ji Chen-feng (Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Commerce, Harbin 150076, China; The Institute of Materia medica and Postdoctoral Programme of HARBin University of Commerce, Harbin 150076, China);Study on the Content Determination and Analysis by HPCE of Polysaccharide from Capparis spinosa Ripe Fruits[A];[C];2006
Chinese Journal Full-text Database 10 Hits
1 WANG Lei 1, YU Rong min 1, ZHANG Hui 1, BIN Wen 1, WANG Yu 1, LI Chun yan 2 , WU Hai yan 2 (1.School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016, China; 2. Shenyang Zhongtian Bioengineering Co., Shenyang 110015,China);Separation, purification and structural analysis of polysaccharides in cultured Cordyceps militaris L.Link[J];Chinese Journal of Biochemical Pharmaceutics;2003-01
2 Zhao Guohua Chen Zongdao 1 Li Zhixiao 2 Kan Jianquan 1 1(Food College, South west Agricultural University, Chongqing,400716) 2(National Key Laboratory of Applied and Organic Chemistry, Lanzhou University, Lanzhou,730000);Advance in Bioactive Polysaccharides[J];Food and Fermentation Industries;2001-07
3 WANG Bin~ 1,3 LIAN Bin~ 1,2 (1.The Provincial Key Laboratory of Fermentation Engineering and Bio-Pharmaceutical,Guizhou University,Guiyang,Guizhou 550003,China;2.Key Laboratory for the Exploitation of Southwestern Resources & the Environmental Disaster Control Engineering,Ministry of Education,Chonqing 400044,China;3.Henan Science and Technology College,Xinxiang,Henan 453003,China);Progress on polysaccharides from edible and medicinal fungi[J];Food and Machinery;2005-06
4 ;超声波法与传统热水法提取灵芝多糖的比较研究[J];Science and Technology of Food Industry;2007-02
5 Yang Juan(16);Study on isolation,purification and purity identification of polysaccharide Le fromLentinus edodes fruitbody[J];SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY;1999-03
6 DONG Cai-hong(Systematic Mycology and Lichenology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China);Review on Advances of Polysaccharides form Cordyceps sinensis[J];Food Science;2004-07
7 HUANG Gui-ping,XIAO Hong,ZHANG Ming-sheng,ZHANG Xiong-lu (Department of Chemistry and Life Science,Gannan Normal University,Ganzhou 341000,China);Study on Extraction of Lentinan with Microwave Technique[J];Food Science;2006-11
8 XIE Hong-qi ZHOU Chun-shan DU Shao-long ZHOU Jing-hua (College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China);Study on Enzymatic Extraction and Ultrafiltration Separation of Lentinan[J];Food Science;2007-04
9 LIU Ning1,2,LI Jian2(1.College of Material Science and Engineering,Northeast Forestry University,Harbin 150036,China;2.College of Food Sciences and Engineering,Harbin University of Commerce,Harbin 150076,China);Study on Comparison of Extraction Methods of Lentinan[J];Food Science;2007-09
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lui Chengmei et al.;Study on Methods to Remove Protein from Lily-Polysaccharides[J];Food Science;2002-01
2 Zhou Hui-Ping; Jiang Xun-Tian; Wang Shu-Ru; Chen QiOng-Hua(Division of Biochemistry, China Pharmaceutical University, Manjing 210009);Effect of Polysaccharide from Enteromorpha prolifera on Lipemia,SOD Activity and LPO Content[J];;1995-02
3 QIU Juan-Ping;SUN Pei-Long;ZHU Jia-Rong;ZHONG Wei-Hong (Zhejiang University of Technology, Hangzhou 310014);STUDY ON MEDIUM OF SUBMERGED CULTURE FOR GRIFOLA FRONDOSA[J];MICROBIOLOGY;2000-04
4 Chen Wuling Cai Xianpeng Shi Jihong Shen Lixin (Department of Biology,Northwest University,Xi'an 710069);Extraction of water soluble polysaccharose from mushroom stem[J];MODERN CHEMICAL INDUSTRY;1998-10
5 LiJun(LiJ) ,HanXianghui(HanXH) ,LiZhonghong(LiZH) ,etal(GansuCollegeofTraditionalChinese Medicine,Lanzhou 730 0 0 );Isolation and deter mination of pachyman in poria cocos(Schw .)wolf[J];CHINESE JOURNAL OF MODERN APPLIED PHARMACY;2000-01
6 Gong Yuntong;Wang Zongchen; Dong Keyin; Ling Minai(Fuzhou Military General Hospital, Fuzhou 350001);The study of lentinan[J];THE JOURNAL OF PHARMACEUTICAL PRACTICE;1996-01
7 Xu Aihua,Chen Huasheng,Wang Ling and Wang Qian (Medical College of Yangzhou University,Yangzhou 225002);Influence of Ginkgo biloba L. Exocarp Polysaccharides on Serum Superoxide Dismutase Activity and Malondialdehyde Level in Mice Under Different States[J];CHINA JOURNAL OF CHINESE MATERIA MEDICA;1998-12
8 Sun Peilong;Jiang Tongjun(Zhejiang University of Technology, Hangzhou 310014);A Review of Eritadenine[J];EDIBLE FUNGI OF CHINA;1995-02
9 Zheng Jianxian;Geng Liping(Dept. of Food Engineering,South China Univ,of Technology);STUDIES ON THE STRUCTURE OF PJFI POLYSACCHARIDE FROM FOOT BODY OF LENTINUS EDODESA[J];EDIBLE FUNGI OF CHINA;1995-05
10 Song Gengping, Xu Aihua, Chen Huasheng, Wang Xiaobin (Medical College of Yangzhou University, Yangzhou 225001);Component Analysis on Folysaccharides in Exocarp of Ginkgo biloba[J];Journal of Chinese Medicinal Materials;1997-09
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved