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《Chinese Journal of Bioprocess Engineering》 2009-02
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Effect of ultrasound on extraction yields,morphology,and biological activity of polysaccharide from Bulpeurum

LI Dai,ZHANG Jing,SUN Run-guang(College of Physics and Information Technology,Shaanxi Normal University,Xi'an 710062,China)  
To study the effect of ultrasound on extraction yields and morphology of polysaccharides from Bulpeurum,single factor tests and orthogonal test were imployed to optimize the extraction process conditions of ultrasound-assisted and the traditional methods.Atomic force microscope(AFM) was used to characterize the morphology of the polysaccharides extracted in the untrasound-assisted process(SBP0) and the polysaccharides extracted by traditional method(WBP0).The phenanthroline-Fe2+ reaction system was used to estimate the activity of Bulpeurum polysaccharide against hydroxyl radicals.The best conditions of ultrasound-assisted extraction were shown as follows: ultrasonic power of 360 W for 15 min,feed to liquid ratio at 1∶35,temperature at 90 ℃ for 1 h.The extraction yield reached 2.58% and the the purity was 44.14%.AFM images showed that the Bupleurum polysaccharides mainly existed in spiral form.Ultrasonic degradation diminished the linear scale of the Bupleurum polysaccharide molecular chains.The scavenging activity against hydroxyl radicals in vitro was positive.The activity of SBP0 was better than that of WBP0 at the same concentration.Within concentration of 80 to 100 μg/mL,the olysaccharide exhibited higher acitivity than that of ascorbic acid.
【Fund】: 国家自然科学基金资助项目(20772077);; 教育部科学技术研究重点资助项目(104167)
【CateGory Index】: S567.79
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