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《Chinese Journal of Bioprocess Engineering》 2014-02
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In vitro biological activity of polysaccharide extracted from Pholiota nameko

LI Jiamei;SUN Runguang;GUO Guoyun;ZHANG Zhi;College of Physics and Information Technology,Shaanxi Normal University;Shaanxi Fashion Engineering University;  
Fungus polysaccharide had an important value of biological activity,Pholiota nameko was plant fungus of edible medicinal value. Water-extracted crude polysaccharides of Pholiota nameko,were investigated. Their antioxidant activity was studied by DPPH method,the lipid peroxidation by Fe2 + method,the antitumor activity by MTT assay,and placenta blue cell count detection. The flow cytometer was used to study effects of PNP on K562 cell cycle. Experimental results showed that PNP had inhibitory ability and DPPH free radical scavenging capability similar to that of Vc on high concentration. PNP had actions on lipid peroxidation with the increasing of the concentration,the inhibitory effect increased. MTT assay showed that PNP inhibited the growth of K562 cells in vitro,exhibited dose-dependent and timebased effect,the highest inhibition rate is 35. 03% when treated with PNP in 800 μg / mL for 48 h. In addition,the result of cell cycle analysis by flow cytometer showed that PNP could block the K562 cell in G1 phase.
【Fund】: 国家自然科学基金(10874108);; 陕西省自然科学基础研究计划(SJ08A16);; 2010国家级大学生创新实验计划(101071812)
【CateGory Index】: R285
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【Citations】
Chinese Journal Full-text Database 8 Hits
1 ZHAO Jun-Xia, ZHENG Li-Fen, ZHAO Juan, SHEN Yuan, WANG Yan-Ling, YAN Yun-Li Department of Cell Biology, Basic Medical College, Hebei Medical University, Shijiazhuang, 050017, China;Effects of pholiota nameko polysaccharides on K562 leukemia cell proliferation and Caspase-3 expression in vitro[J];Journal of the Fourth Military Medical University;2007-15
2 ZHANG Zhi-jun,LI Shu-fang,WEI Xue-sheng,CHEN Xiao-ming(The Forestry and Fruits Institute of Tianjin,Tianjin 300112,China);Study on Antioxidant Activity of Ganoderma Lucidum Polysaccharide[J];Chemistry & Bioengineering;2011-03
3 WEI Wen-qing,ZHAO Man-cang,LIU Jing,JIAO Juan,ZHANG Yan,FU Yao.Department of Central Laboratory,General Hospital of Beijing Military Command,Beijing 100700,China;Methodological studies on the anti-cancer drug sensitivity of cancer cells with MTT colormetric assay[J];Chinese Clinical Oncology;2008-10
4 Gou Mingyue,Liu Liang,Zhang Chunzhi(Dalian Polytechnic University,College of Bio & Food Technology,Dalian 116034,China);Determination of Antioxidant Activity in 26 Plants by DPPH Method[J];Food and Fermentation Industries;2010-03
5 Zhang Erxian et al.;Study on Anti-oxidative Activity of Flos Chrysanthemum Extract[J];FOOD SCIENCE;2000-07
6 CHEN Yi~1,XIE Ming-yong~(1*),GONG Xiao-feng~(1,2)(1.Key Laboratory of Food Science of Ministry of Education,Nanchang University,Nanchang 330047,China;2.School of Environmental Science and Engineering,Nanchang University,Nanchang 330029,China);DPPH Radical Scavenging Effect of Ganoderma atrum[J];Natural Product Research and Development;2006-06
7 XIANG Ying,CHEN Jian*(College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China);Analysis and Evaluation of Nutritional Components in Fruit Body of Pholiota nameko[J];Food Science;2013-06
8 DU Qing (Chongqing Urban Management Professional College,Chongqing 401331,China);Research Advancement of Edible (Medicinal) Fungi Polysaccharides[J];Food and Nutrition in China;2011-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Hong et al (Department of Chemistry,Hainan Normal University,Haikou,Hainan 571158);Study on the Antioxidation of the Flowers Extracts from 15 Ordinary Tropical Plants[J];Journal of Anhui Agricultural Sciences;2008-01
2 LI Dong-ling(Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing,Jiangsu 210014);Comparative Analysis on Essential Oil and Total Flavonoids of Medicinal Chrysanthemum from Different Original Locations and Species[J];Journal of Anhui Agricultural Sciences;2010-07
3 PAN Ke-ji(Department of Life Sciences,School of Yuanpei,Shaoxing University of Arts and Sciences,Shaoxing,Zhejiang 312000);Optimization of the Preparation Technology of Chrysanthemum Oral Solution by Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2012-12
4 DUAN Chong-xia,ZHANG Zheng-zhu(Key Laboratory of Tea Biochemistry & Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036);Comparison of the functional constituents in four kinds of medicinal Flos chrysanthemi[J];Journal of Anhui Agricultural University;2008-01
5 MA Shi-tang,ZHANG Xiao-Lin,YU Hao,ZHOU Guo-liang,LIU Han-zhen(College of Food and Drug,Anhui Science and Technology University,Fengyang 233100,China);Study on Antitumor Activity of Thiadiazoles Heterocycle Compounds Against K562 Cell[J];Journal of Anhui Science and Technology University;2012-02
6 YU Li-li,LIU Hong-zhang,ZHU Mei,ZHANG Shuang (College of Life Sciences,Jilin Agricultural University,Changchun,Jilin 130000);Study on Antioxidant and Antibacterial Activity of Ethanol Extract of Ligularia jaluensi[J];Northern Horticulture;2012-12
7 Zheng Feixia~*,Lin Zhongdong Department of Neurology,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Zhejiang(325027),China;Study on Protective Effects of Ganoderma Capense on Febrile Seizures in Rats[J];Journal of Zhejiang Chinese Medical University;2011-05
8 ZHAO Jin,LAI Ji-xiang,HE Cong-fen,ZHAO Hua,YANG Ya-dong,DONG Yin-mao(Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China);Extraction technology of active ingredients in plant Rhodiola and its function in cosmetics[J];China Surfactant Detergent & Cosmetics;2009-06
9 PENG Li-wei1,HE Cong-fen1,ZHAO Hua1,ZHU Jun1,LIU Guang-rong2,DONG Yin-mao1(1.Beijing Key Lab of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China; 2.Infinite Extremely(China) Co.,LTD,Guangzhou,Guangdong 510665,China);Study of change of content of skin-care active ingredients in soybean during its germination process[J];China Surfactant Detergent & Cosmetics;2011-02
10 SONG Xiao-feng1,YUAN Zeng-yan2,XU Ping-hui1(1.Xinxiang Biocaro Co.,Ltd,Xinxiang,Henan 453300,China; 2.Sanquan Medical College,Xinxiang,Henan 453000,China);Extraction of active ingredients in Honey suckle and their function in cosmetics[J];China Surfactant Detergent & Cosmetics;2012-02
China Proceedings of conference Full-text Database 1 Hits
1 JIANG Yan,ZHENG Li,Kerimjan·TURSUNJAN (College of Life Science and Technology,Xinjiang University,Urumqi 830046,China);Antioxidative Effects in vitro of Anthocyanins from Morus nigra L.[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 JIANG Ping-ping1,2,HAN Ye1*,GU Sai-hong2,ZHANG Wei-ping2,XING Zeng-tao2(1.College of Agriculture and Bio-engineering,Tianjin University,Tianjin 300072;2.Institute for Agro-food Standards and Testing Technology,Shanghai Academy and Agricultural Sciences, Shanghai 201106,China);Determination of Amino Acids in Five Edible Fungi and Their Nutritional Evaluation[J];Amino Acids & Biotic Resources;2009-02
2 LIU Jian-hua,ZHANG Zhi-jun (Forestry and Pomology Institute of Tianjin,Tianjin300112,China);Progress of Fxtraction and Application of Ganoderma Amylose[J];Storage and Process;2003-03
3 JIA Lin Tao, YU Cui Juan, XU Yan Ming, ZHU Feng, PENG Wei Dan, SU Cheng Zhi, WANG Cheng Ji, YANG An Gang Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China;Construction and effect of caspase-3 fusion proteins and their apoptosis induction in HeLa cells[J];Journal of The Fourth Military Medical University;2001-12
4 YU Xiao-Lin, LIU Hong, CHEN Wan-Ping, CHENG Peng-YuanOutpatient Department, Fourth Military Medical University, Xi'an 710033, China;Effects of recombinant Caspase-3 on human laryngocarcinoma cell line Hep-2 through coexpression with GFP[J];Journal of The Fourth Military Medical University;2005-21
5 LI Zhi-min,WANG Bo-chu,ZHOU Jing, PENG Liang nistry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China);Comparison of three methods of removing protein from polysaccharide extract in the plant[J];Journal of Chongqing University(Natural Science Edition);2004-08
6 Chen Jin-yan (Putian Branch of Fujian Medical Uuiversity, Putian, Fujian, 351100,china) Lu Zheng-hui (Fujian Research Institute of Light Industry, Fuzhou, Fujian, 350005, china);Discussion on the immune response and antitumor mechanism of mycopolyose[J];The Light & Textile Industries of Fujian;2002-04
7 ZHANG Zun-ting , HE Yun, LIU Qian-guang (School of Chemistry and Materials Science, Shannxi Normal University, Xi′an 710062) and YANG Bo-lun (College of Environment and Chemical Engineering, Xi′an Jiaotong University, Xi'an 710049);Spectrophotometric determination of antioxidative activity of 20 kinds of Chinese traditional and herbal drugs in the Tai Bai mountain[J];Analytical Laboratory;2002-02
8 ZHANG Xue-hong1, HU Hong-bo1, TANG Yong-lian1, HUANG Rui-shan1, CHEN Zheng-jie2 (1. School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China; 2. Zhejiang Hisun Pharmaceutical Co. Ltd, Taizhou 318000, China);Continuous Fermentation of Ganoderma lucidum Exo-polysaccharide in a Membrane Bioreactor[J];Journal of Chemical Engineering of Chinese Universities;2002-06
9 Hu chun;Optimization of Technology for Flavonoid Extraction from Chrysan themum[J];Guangzhou Food Science and Technology;1996-02
10 WU Xin-jun~(1,2), MAO Pei-hong~(1,3), JIN Xiang~(1,3),ZENG Xian-xian~(1,3) (1. Ion Beam Bio-Engineering Center, Xinjiang University, Urumqi 830008, China; 2. College of Chemistry and Chemical Industry, Xinjiang University, Urumqi 830046, China; 3. Department of Physics, Xinjiang University, Urumqi 830046 , China);Study on Polysaccharide from Edible and Medical Fungus[J];;2004-01
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