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《Chinese Journal of Bioprocess Engineering》 2014-02
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In vitro biological activity of polysaccharide extracted from Pholiota nameko

LI Jiamei;SUN Runguang;GUO Guoyun;ZHANG Zhi;College of Physics and Information Technology,Shaanxi Normal University;Shaanxi Fashion Engineering University;  
Fungus polysaccharide had an important value of biological activity,Pholiota nameko was plant fungus of edible medicinal value. Water-extracted crude polysaccharides of Pholiota nameko,were investigated. Their antioxidant activity was studied by DPPH method,the lipid peroxidation by Fe2 + method,the antitumor activity by MTT assay,and placenta blue cell count detection. The flow cytometer was used to study effects of PNP on K562 cell cycle. Experimental results showed that PNP had inhibitory ability and DPPH free radical scavenging capability similar to that of Vc on high concentration. PNP had actions on lipid peroxidation with the increasing of the concentration,the inhibitory effect increased. MTT assay showed that PNP inhibited the growth of K562 cells in vitro,exhibited dose-dependent and timebased effect,the highest inhibition rate is 35. 03% when treated with PNP in 800 μg / mL for 48 h. In addition,the result of cell cycle analysis by flow cytometer showed that PNP could block the K562 cell in G1 phase.
【Fund】: 国家自然科学基金(10874108);; 陕西省自然科学基础研究计划(SJ08A16);; 2010国家级大学生创新实验计划(101071812)
【CateGory Index】: R285
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