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Cloning and expression of endostatin in escherichia coli

WU Cheng-jun1,YE Li-jian 1,PENG Xiao-zhong 3,MA Zhi-gang 1, LIU Ji-mei 1,HU Ning-zhu 2,YAN Bo 1,HUANG Xin-qi 1 (1.Biotechnology Research Institute Yunnan Academy of Agricultural Science,Kunming 650223,China;2.Institute of Medical Biology CAMS,K  
The total RNA was extracted from the fetal liver and the endostatin gene was amplifieated by RT-PCR.After identified by DNA sequencing,it was cloned into the expression vector pGEX-KG and then transformed into the host cells E.coli DH5α and BL21.Subsequently the recombinant GST-fusion protein was expressed and purified.
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