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《Biotechnology》 2004-02
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The Analysis Based on DNA Sequences from ITS Ribosomal DNA of Indica and Japonica Subspecies in Rice

GAO Jian~1,LIANG Man-zhong~2,CHEN Liang-bi~(2*) (1.College of Life Science,Hunan Science and Technology University,Xiangtan 411201;2.College of Life Science,Hunan Normal University,Changsha 410081)  
The entire ITS region (including 5.8S rDNA)of nuclear ribosomal DNA of six cultivated varieties i.e.three indica varieties (Nanjing11,9311,Guanglu′ai4) and three japonica varieties (Qiuguang,Ribenqing,Zhaowadao) was amplified using PCR technique and sequencedwith automated fluorescent sequencing in order to approach the genetic background and predestined relationship between indica and japonica subspecies in rice.The result showed that the 5.8S rDNA length and G/C content of six experimented varieties were completely the same;the sequences of ITS1 ranged from 193bp to 195bp in size and G/C content varied from 72.31% to 74.38%;the sequences of ITS2 ranged from 224bp to 232bp in size and G/C content varied from 74.46% to 76.86%; the similarity of sequences varied from 94.4% to 99.3%.The alignment and analysis by CLUSTAL W software revealed:①there were four informative sites that divided the 6 experiment varieties into indica and japonica groups.②the homologue of ITS sequences in indica varieties was smaller than that of ITS sequences in japonica varieties.Thus it can be seen that the varying regularity of ITS sequences in rice has a high concordance with its traditional classification.
【CateGory Index】: S511.2
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