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《Biotechnology》 2004-02
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Study on Plasmid Stability of Recombinant Anabaena sp.PCC7120

LIU Zhi-wei~(1,2),ZHANG Chen~2,GUO Yong~1 (1.Department of Biology,Jia Ying University,Meizhou 514015;2.College of Food Engineering and Biotechnology,South China University of Technology,Guangzhou 510640,P.R.China)  
The plasmid stability of recombinant Anabaena sp.PCC7120 was studied.The experiment proved that recombinant Anabaena sp.PCC7120 could remain segregative stability of recombinant plasmid pDC-TNF.Through comparing the growth and exogenous gene expression of recombinant Anabaena sp.PCC7120 that existed from generation to generation without neomycin and cultured in different medium,it is also proved that the structural stability of recombinant plasmid pDC-TNF was high,but the copy number of recombinant plasmid pDC-TNF would reduce if recombinant Anabaena sp.PCC7120 was cultured in medium without neomycin.The neomycin in the seed medium could hold the stability of pDC-TNF during culture process,so the quantity of neomycin used in fermentation culture could be reduced much.
【Fund】: 广东省自然科学基金资助项目 (“转基因海藻的构建及其培养技术研究” 980 541 )
【CateGory Index】: Q819
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