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《Biotechnology》 2007-06
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Protoplast Preparation and Regeneration of Pleurotus cornucopiae

YANG Feng-ling,JIA Le,REN Xuan(College of Life Science,Shandong Agriculture University,Tai'an 271018,China)  
Objective:The optimum conditions of isolation and regeneration of protoplasts from the mycelia of Pleurotus cornucopiae were studied.Methods:Maximum yield of protoplasts could be obtained with 5d-mycelia by using enzyme system composed of 1.5% lywallzyme and 0.5% cellulase with 0.6mol/L mannitol being used as osmotic stabilizer at 30℃ for 2.5h.Results:The highest protoplast yield and regeneration rate of Pleurotus cornucopiae was 3.12×107 and 0.78% on RM2.The compotent of this medium was potato 200g,sucrose 20g,yeast extracts 2g,KH2PO4 0.3g,MgSO4·7H2O 1.5g,vitamin B1 0.1g,0.6mol/L mannitol as osmotic stabilizer.These results would provide important parameters for the strain improvement of Pleurotus cornucopiae through the protoplast technique.
【Fund】: 山东省自然基金项目资助(Y2006D08)
【CateGory Index】: S646
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