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《Biotechnology》 2008-04
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Study on Extraction and Anti-microbial Activities of Total Flavonoid from Equisetum ramosissimum

LI Guo-qing1,Ainiwaer·AIKEMU2,LI Jia1,Sultan ABABARRI1(1.College of Life Science and Technology, Xinjiang University, Xinjiang 830046, China;2.Xinjiang Medical University Pharmacy Department Analysis Office, Xinjiang 830046, China)  
Objective:Extraction of the flavonoid from the aerial of Equisetum ramosissimum with different method was studied in this paper, and the in vitro antimicrobial activity of the flavonoid from the samples against bacteria is also assayed and reported.Method:The different methods were compared and the extraction was treated by means of color reaction, precipitation reaction and antibacterial experiments. Result:Total flavonoid extracted rate was 0.749% by Soxhlet extraction (SE). Ultrasonic-assisted extraction were secondary. The color reaction was positive, and there were obvious sediment in the precipitation reaction and obvious inhibition zone in the antibacterial experiments as well. Conclusion: Ultrasonic-assisted extraction is an optimal method to extract the flavonoid from Equisetum ramosissimum; the flavonoid in Equisetum ramosissimum can suppress Escherichia coli and Sarcinasp, powerfully.
【Fund】: 新疆少数民族科技人才特殊培养计划项目资助(2003-10)
【CateGory Index】: S567.239
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