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《Biotechnology》 2009-03
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Application of PCR-SSCP Technology in Diversity Analysis of Microbial Communities

WANG Yan1,SHEN Xi-quan2,WU Zu-fang1,2,WENG Pei-fang2(1.Key Laboratory of Applied Marine Biotechnology,Ministry of Education;2.Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China)  
PCR-SSCP analysis method is a newly developed molecular biological technology,and has its broad and potential application value with its many advantages such as rapid,simplified,sensitive analysis and its suitable screening for large samples,in the meanwhile,it is an effective way to detect the gene variation of base replacement,deletion and insertion,etc.The development of PCR-SSCP method,technological characters and its application of microbial community diversity analysis in the fields of environmental ecology as well as food microbe,etc.were elucidated in this paper.Its future prospect using PCR-SSCP technology was also described.
【Fund】: 浙江省自然科学基金项目(Y3080231);; 宁波市自然科学基金项目(2007A610054)资助
【CateGory Index】: Q93-33
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【References】
Chinese Journal Full-text Database 4 Hits
1 PAN Qin-chun~(1,2),MENG Zhen~1,ZHONG Qi-ding~1,XIONG Zheng-he~1,ZHANG Yu-bin~2 and WU Zhao-zheng~3 (1.China National Research Institute of Food & Fermentation Industries,Beijing 100027;2.School of Life Sciences & Technology of China Pharmaceutical University,Nanjing,Jiangsu 210009;3.Gubeichun Group Co.Ltd.,Wucheng,Shandong 253300,China);Investigation on the Application Foreground of Molecular Ecology Technique in the Study of Daqu Microbial Community Diversity[J];Liquor-Making Science & Technology;2011-03
2 WU Zhao-hai,XU Qing-fang(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China);Research and Application Development of PCR-SSCP Technology[J];Animal Husbandry and Feed Science;2010-08
3 LIANG Wei1,WU Suqing1,2,WU Zhenbin1 1.State Key Laboratory of Freshwater Ecology and Biotechnology//Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China.2.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China;The application of molecular techniques to characterize the microbial communities in constructed wetland[J];Ecology and Environmental Sciences;2010-04
4 Zhang Rui Wu Zufang Shen Xiquan Lei Lanlan (Key Laboratory of Applied Marine Biotechnology,Ministry of Education & Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211);Microbial Community Structure and Its Dynamic Analysis during the Processing of Low-salinity Pickled Mustard Tuber[J];Journal of Chinese Institute of Food Science and Technology;2011-03
【Citations】
Chinese Journal Full-text Database 8 Hits
1 Dong Chengliang Deng Changyan (The College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070);Progress of Examination Techniques for Gene Mutation[J];JOURNAL OF YELLOW CATTLE SCIENCE;1998-03
2 ZHAO Yang-guo,WANG Ai-jie,REN Nan-qi,ZHAO Yan(School of Environmental and Municipal Engineering,Harbin Institute of Technology,Harbin 150090,China);Microbial Community Structure in Different Wastewater Treatment Processes Characterized by Single-Strand-Conformation Polymorphism(SSCP) Technique[J];Environmental Science;2006-07
3 LIU Xiao-lin,LIU Wen-jun(Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China);Microbial Community Structure in Bio-Ceramics and Biological Activated Carbon Analyzed by PCR-SSCP Technique[J];Environmental Science;2007-04
4 SHANG Li-qin1,BAI Lin*,TAN Bing,LIU Xiu-fei,ZHANG Hong-tao(College of Animal Science and Technology,Sichuan Agricultural University,Ya 'an Sichuan 625014);Study on Microbial Community Structure in Earthworms Faecal by Single Strand Conformation Polymorphism(SSCP) Technique[J];Acta Ecologiae Animalis Domastici;2008-02
5 XIE Bing,DONG Liang (Department of Environmental Science,East China Normal University,Shanghai 200062,China);Study on Microbial Community Structure of Reed Constructed Wetland Using PCR-SSCP[J];System Sciences and Comprehensive Studies in Agriculture;2007-02
6 Li Yumei1 Yao Jiyuan2 Wu Jing2 Bai Xiujuan1(1Northeast Agriculture University Heilongjiang,Harbin 150030;2Jilin Agriculture University,Changchun 130118);Research and Application Development of PCR-SSCP Technology[J];Biotechnology Bulletin;2007-06
7 XUE Yan,CHANG Hong,CHANG Guo-bin((College of animal science and technology,Yangzhou University 225009);PCR-SSCP Technique and Its Advances in Research of Animal Breeding[J];Journal of Animal Science and Veterinary Medicine;2005-03
8 WANG Yan-yan,ZHANG Hui,XIANG Wen-sheng(1.College of Life Science,Northeast Agricultural University,Harbin 150030,China;2.Insitute of Crop Germplasm Resources,Chinese Academy of Agricultural Sciences,Beijing 100081,China);PCR-SSCP Technique and Its Application in Botanical Study[J];China Journal of Bioinformatics;2008-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liang Ning,Wang Dunqiu,Wei Caichun (Guilin University of Technology,Guilin 541004,China);An Assessment of Microorganisms in the Constructed Wetland[J];Environmental Science and Management;2010-02
2 ZOU Li,HUANG Yi,XIE Shuguang College of Environmental Sciences and Engineering,Peking University,Beijing 100871;Study on Bacterioplankton Community in Wujin River[J];Acta Scientiarum Naturalium Universitatis Pekinensis;2011-03
3 LI Hong-hao,REN you-she,YUE Wen-bin(College of Animal Science and Technology,Shanxi Agricultural University,Taigu Shanxi 030801,China);PCR-SSCP Technique Advances and Its Application in Economic Traits Research of Goat and Sheep[J];Grass-Feeding Livestock;2006-01
4 Zhao Zhao-xia1,Liu Wen-zhong1,Zhu Wen-jin2,et al(1. College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China;2. Department of Animal Science,Hebei Normal College of Science and Technology);Analysis on the Genetic Diversity of Partial Sequences in Mitochondrial DNA D-loop of Six Donkey Breeds[J];China Herbivores;2008-04
5 Xiang Hong1,2,3 Lü Xiwu1 Yin Lihong2 Zhu Guangcan1 Liu Ran2 Su Yaoyao2 Yang Fei2(1 School of Energy and Environment,Southeast University,Nanjing 210096,China)(2School of Public Health,Southeast University,Nanjing 210009,China)(3Guizhou Provincial Center for Disease Prevention and Control,Guiyang 550004,China);Microbial community structure and predominant bacteria in water purification process[J];Journal of Southeast University(Natural Science Edition);2010-03
6 ZHOU Ming-tao,GAO Hong,XIAO Peng,ZHOU Hui(College of Animal Science Technology,Yunnan Agricultural University,Kunming,Yunnan,650201,China);Advance in PCR-SSCP and Its Application[J];Progress in Veterinary Medicine;2009-06
7 MENG Han1,HUI Wei2,XIAO Yi-ping1,TIAN Guan-rong2,QUAN Zhe-xue1 (1.Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University Shanghai 200433,China;2.College of Science,Yanbian University,Yanji 132300,China);Comparison of Cultured Microbial Diversity in Chongming Dongtan Soils Covered by Different Plants[J];Journal of Fudan University(Natural Science);2010-01
8 CAI Chen-qiu et al(College of Life and Environmental Sciences,Minzu University of China,Beijing 100081);Soil Microbial Diversity and Its Research Methods[J];Journal of Anhui Agricultural Sciences;2011-28
9 Zhang Wei1,2 Zhao Jun1,2 Lang Xianming1,2 Li Xiaodong1,2 Kuang Deshun3(1.Liaoning Beifang Environmental Protection Co.Ltd.,Shenyang 110031,China;2.Liaoning Key Laboratory of Basin Pollution Control,Liaoning Academy of Environmental Science,Shenyang 110031,China;3.College of Land and Environment,Shenyang Agricultural University,Shenyang 110161,China);Progress in studies on pollutant removal by microorganisms in constructed wetlands[J];Chinese Journal of Environmental Engineering;2010-04
10 JIA Cai-yun,LIU Hong-yu,ZENG Guang-ming,ZHANG Lin-da,WU Jin-zhuangCollege of Environmental Science and Engineering,Hunan University,Changsha 410082,China;Studies on Isolation and Characterization of Gasoline Degrading Bacteria[J];Research of Environmental Sciences;2008-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YAO Cheng-qiang (Logistic Service Group Yangtze Normal University,Chongqing 408100);Manufacturing Technology of Packet Mustard with Low Salt and without Preservative[J];Journal of Anhui Agricultural Sciences;2008-19
2 HU Shu-fang et al(Key Laboratory of Ion Beam Bio-engineering in Henan Province,Zhengzhou University,Zhengzhou,Henan 450052);Study on the Seperation and Identification of Lactic Acid Bacteria from Natural Fermented Pickle and Their Physiological Characteristics[J];Journal of Anhui Agricultural Sciences;2009-15
3 YANG Rong,SU Yong-zhong(Laboratory of Watershed Hydrology and Ecology,CAREERI,CAS,Linze Inland River Basin ComprehensiveResearch Station,CERN,Lanzhou Gansu 730000,China);Groundwater Nitrate Pollution in the Oasis Agricultural Areas in the Middle Reaches of Heihe River,Northwest China[J];Journal of Glaciology and Geocryology;2008-06
4 LIU Xiao-bo1,KANG Shi-chang1,2,LIU Yong-qin1,HAN Wen-wu1(1.Laboratory of Tibetan Environment Changes and Land Surface Processes(TEL),Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100085,China;2.State Key Laboratory of Cryospheric SciencesCAREERI,CAS,Lanzhou Gansu 730000,China);Microbial Community Structures in the Nam Co Lake,Tibetan Plateau,and Comparison with Other Alpine Lakes[J];Journal of Glaciology and Geocryology;2008-06
5 ZHOU Guo-hua~1,WAN Yan-hua~2,LIU Yan-qiu~3(1.2 School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou?730070,China;3.The Library of Jingdezhen Ceramic Institute,Jingdezhen?333001,China);Ammonia-Nitrogen and Its Treatment in Micro-Polluted Water Source[J];Environmental Science and Management;2006-06
6 Zhang Xiaohui~1 Li Desheng~1 Sun Wenjun~2 (1.The Environment and municipal engineering College of Lanzhou Jiaotong University,Lanzhou 730070,China;Department of Environment and Technology Tsinghua,Beijing 100084,China);Study on the Removal Effect of NH_4-N by the Biological Manganese Sand Filter Formed Naturally[J];Environmental Science and Management;2007-05
7 Li Huai1,Guan Weisheng1,Ou Yangerming2,Wang Xiaohui2,Zhang Jie2(1.Department of Environmental Science and Engineering,Chang'an University,Xi'an 710054,China;2.Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China);DGGE(Denatured Gradient Gel Electrophoresis) and its Application in the Research of Environmental Microbiology[J];Environmental Science and Management;2008-10
8 WANG Jun~(1,2),XIE Hao~(3),GUO Er-hu~(2),LI Ai-jun~(2),FAN Hui-ping~(2)(1.China Agricultural university,Beijing 100094;2.Institue of Foxtail Millet, Shanxi Academy of Agricultural Sciences,ChangZhi 046011;3.Beijing Agricultural College, Beijing 102206);DNA Molecular Marker and Its Application on the Heredity and Breeding Research in Foxtail Millet[J];Journal of Beijing Agricultural College;2005-01
9 GAO Xue 1, XU Shang zhong\+2, ZHANG Ying han 1(1. College of Animal Science and Technology, Northwest Sci Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100,China(2. Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100094,China);Advomce in Action Mechanism of Growth Hormone[J];Journal of Yellow Cattle Science;2003-01
10 A case in the north high plain of Songhua River in Songnen BasinDONG Wei-hong~(1,2), LIN Xue-yu~(1,2) (1.College of Environment and Resources, Jilin University, Changchun130026, China;2.Institute of Water Resources and Environment, Jilin University,Changchun130026,China);Analysis on the influence factors of the nitrogen pollution in shallow groundwater[J];Journal of Jiling University (Earth Science Edition);2004-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LUO Hui-bo,HOU Hai-bo,HUANG Zhi-guo,WEI Chun-hui,YE Guang-bin(Liquor-making Biotechno logy & Application key Laboratory of Sichuan province,Sichuan University ofScience & Engineering,Zigong 643000,China);Optimization of PCR-DGGE Electrophoresis Conditions of Eucaryotic Microorganisms Community in Daqu[J];Journal of Sichuan University of Science & Engineering(Natural Science Edition);2011-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHONG Hua ① LAI Xu Long ② WEI Rong Ping ③ LIU Zhong Lai ①** (① College of Life Science,Central China Normal University,Wuhan 430079, China) (② Faculty of Earth Sciences,China University of Geosciences,Wuhan 430074, China) (③ China Conservation and Research Center for the Giant Panda,Wolong 623006, Sichuan, China);An improved protocol for DNA extraction from the faeces of the giant panda[J];Acta Zoologica Sinica;2003-05
2 ZHANG Bao Wei,WEI Fu Wen ** ,LI Ming,L U ·· Xiao Ping Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China;A simple protocol for DNA extraction from faeces of the giant panda and lesser panda[J];Acta Zoologica Sinica;2004-03
3 Dong Chengliang Deng Changyan (The College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070);Progress of Examination Techniques for Gene Mutation[J];JOURNAL OF YELLOW CATTLE SCIENCE;1998-03
4 SHIYue1,RENNanqi1,YANXianfeng1,DINGJie1,WANGJinqu*2,GAOYu1( 1.School of Municipal & Environ. Eng., Harbin Inst. of Technol., Harbin 150090, China;2.School of Chem. Eng., Dalian Univ. of Technol., Dalian 116012, China );Full-scale studies of biological treatment of wastewater from traditional Chinese medicine production[J];Journal of Dalian University of Technology;2003-04
5 WANG Ai-jie;REN Nan-qi;LIN Ming;DU Da-zhong, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China, E-mail: waj05782003@163. com;Stabilization and control of alkalinity during sulfate-reduction[J];;2003-06
6 WANG Ai jie,REN Nan qi, LIN Ming, DU Da zhong (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China,E mail:waj05782003@163.com);Stabilization and control of alkalinity during sulfate-reduction[J];Journal of Harbin Institute of Technology;2003-06
7 HAO Gui-yu, HUANG Min-sheng, XU Ya-tong (Department of Environmental Science & Technology,East China Normal University,Shanghai200062,China);Earthworms and Their Application to Eco-Environmental Protection[J];Research of Environmental Sciences;2004-03
8 Zuo Jian-eHu Ji-cui (Department of Environmental Engineering, Tsinghua University, Beijing);Anaerobic Biological Treatment of Organic Wastewater Containing Sulfate[J];Environmental Science;1991-03
9 ZHAO Yang-guo, REN Nan-qi, WANG Ai-jie, LIU Guang-min, ZHAO Qiu-shi, SHANG Huai-xiang (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Microbial Community Structure Analyzed by Single-Strand Conformation Polymorphism Technique in Sulfate-Reducing Reactor[J];Environmental Science;2005-04
10 ZHAO Yang-guo,WANG Ai-jie,REN Nan-qi,ZHAO Yan(School of Environmental and Municipal Engineering,Harbin Institute of Technology,Harbin 150090,China);Microbial Community Structure in Different Wastewater Treatment Processes Characterized by Single-Strand-Conformation Polymorphism(SSCP) Technique[J];Environmental Science;2006-07
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