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《Biotechnology》 2010-01
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Separation,Purification of Cu,Zn-SOD from Goat Blood

GAO Wei,SUN Qing-lin(College of Bioengineering,Inner Mongolia Agricultural University,Hubhot 010018,China)  
Objective:To extraet SOD from goat blood.Method: Cu,Zn-SOD was purified from goat blood by organic solvent to remove hemoglobinn,thermal denaturalization,super-filtration condensation,acetone precipitation and DEAE-32 ion-exchange chromatography.using non-denaturing gel electrophoresis,stained with NBT activity and its molecular weight was analyzed by the SDS-PAGE.Result:The 1 000ml goat blood has been SOD powder 1 257mg,the total activity was 79 453U,specific activity was 3 871.4U/mg·pr.Electrophoresis showed that SOD has two SOD activity bands.Through SDS-PAGE electrophoresis determination of the two SOD subunits proteins with molecular weight were 16.71kDa,15.97kDa.The purified goat blood SOD activity inhibition by H2O2,Through UV scanning,there was goat blood SOD in the 230.5nm absorption peak.
【CateGory Index】: Q814
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