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《Biotechnology》 2010-05
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Optimization Culture of Vibrio harveyi EcGY020401

TAO Jia-fa,LAI Ying-tiao,PAN Hou-jun,SHI Cun-bin,WU Shu-qin(Pearl River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory of Aquatic Animal Immune Techniques,Guangzhou 510380,China)  
Objective:It was offered data to produce bacterin of V.harveyi.Method:It was cultured by vibrating or fermenting,CFU/mL of culture was mensurated by plate counting.Result:The results were showed that the NaCl content of 20-25g/L was the most suitable for the growth.The most suitable pH and the glucose content for the growth were 7.5-7.7 and 2-5g/L respectively.The component of TSB medium was optimized,The component of medium improved:tryptone 5 g/L,peptone 15g/L ,soyptone 3g/L,yeast extract 1g/L,glucose 4g/L,K2HPO4 5g/L,NaCl 15g/L,pH 7.5,It was 2.95×1010cfu/mL for cultured strain of EcGY020401,It was needed 10 h to attain the most biologic capacity with 5L ferment vessel,maximum concentration of 3.46×1010cfu/mL.Conclusion:It was cultured strain of EcGY020401 to use TSB medium improved with ferment vessel.it could be reduced cost and increased culturing efficiency.
【Fund】： 国家“863”计划重点项目(“海水养殖鱼类细菌性疾病亚单位疫苗的研制与应用” 2001AA622050);; 广东省重点科技计划项目(“养殖鱼类重要疾病疫苗研制” B205)资助
【CateGory Index】： Q93-335
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【References】
Chinese Journal Full-text Database 1 Hits
1 WU Shuqin;TAO Jiafa;GONG Hua;CHANG Ouqin;REN Yan;LIU Lihui;CHEN Zonghui;LAI Yingtiao;Key Laboratory of Fishery Drug Development,Ministry of Agriculture; Key Laboratory of Aquatic Animal Immune Technology,Pearl River Fishery Research Institute,Chinese Academy of Fishery Sciences;;Situation and tendency of the fishery vaccine development[J];Chinese Fishery Quality and Standards;2014-01
【Citations】
Chinese Journal Full-text Database 10 Hits
1 MAO Zhi-juan~1, LOU Dan~1,WU Jian-jun~2 (1.Ningbo Fisheries Research Institute, Ningbo 315012,China;2. Huazhong Agricultural University, Wuhang 430070,China);Studies on Culture Technological Conditions of Vibrio harveyi Y91301[J];Fisheries Science;2004-03
2 Ma Yue, Zhang Yuanxing, Sun Xiuqin  (State key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237) ( First Institute of Oceanography, State Oceanic Administration, Qingdao 266003);Production of Marine Fish Vaccine for Vibriosis from Vibrio anguillarum: Optimization of New Culture Medium for High density Culture[J];High Technology Letters;2001-07
3 CHEN Shi-Yong,ZHANG Pei-Jun ,MO Zhao-Lan ,ZOU Yu-Xia ,ZHANG Zhen-Dong ,XU Yong-Li  (Experimental Marine Biology Laboratory,Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071;Graduate School,Chinese Academy of Sciences,Beijing,100039)  (Experimental Marine Biology Laboratory,Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071);THE IMPACT OF CULTURE CONDITIONS ON VIBRIO ANGUILLARUM STRAIN M3 GROWTH AND PROTEASE PRODUCTION[J];Oceanologia Et Limnologia Sinica;2004-02
4 FANG Hai1 QIN Guo-min2 CHEN Cui-zhen1 ZHANG Xiao-jun2 GE Mu-xiang1(1 Key Laboratory of Perventive Veterinary Medicine of Hebei, Hebei Normal University of Science and Technology,Qinhuangdao 066004) (2 Key Laboratory of Oceanic Biotechnology of Jiangsu, Huaihai Institute of Technology, Lianyungang 222005);Biological characteristics of Vibrio harveyi from diseased oceanic whitetip shark Carcharhinus longimanus L.[J];Progress in Fishery Sciences;2009-01
5 MA Aimin1,2,YAN Maocang1,XIE Qilang1,CHEN Shaobo1,SHAN Lezhou1,CHANG Weishan2(1.Zhejiang Mariculture Research Institute,Wen zhou 325005,China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Wenzhou 325005,China;3.Department of Animal Sciences and Technology,Shandong Agricultural University,Tai’an,271018,China);Studies on the Optimum Culture Conditions of Vibrio splendidus M-1[J];Bulletin of Science and Technology;2009-06
6 PAN Xiao-yi1,SHEN Jin-yu1,YIN Wen-lin1,et al(1.Zhejiang Institute of Freshwater Fisheries, Huzhou 313001,China);Studies on the Optimal Culture Conditions of Vibrio harveyi BK-1 from Sparus macrocephalus[J];Journal of Zhejiang Ocean University;2005-03
7 LI Yu-ying, LI Jin-nian, YU Wei-yi (College of Husbandry and Fisheries,Anhui Agricultural University,Hefei230036,China);Selection of optimal producing condition of the exotoxin of Vibrio mimicus from Eriocheir sinensis[J];Journal of Fisheries of China;2003-05
8 WU Hou-Bo and PAN Jin-Pei (South China Sea Institute of Oceanology, CAS, Guangzhou 510301);EFFECTS OF CULTURAL CONDITIONS ON THE PRODUCTION OF TOXIN BY VIBRIO MIMICUS[J];Acta Hydrobiologica Sinica;2003-02
9 Shen Jinyu Pan Xiaoyi Yin Wenlin Hao Guijie Cao Zheng (Zhejiang Institute of Freshwater Fisheries,Huzhou 313001);Studies on the Optimal Culture Conditions of Vibrio harveyi GYC1108-1 from Pseuclosciaena crocea[J];Biotechnology Bulletin;2009-S1
10 LIU Dai-Xin1 NING Xi-Bin1 ZHANG Ji-Lun2(1.College of Food Science,Shanghai Fisheries University,Shanghai 200090)(2.Shanghai Entry-exit Inspection and Quarantine Bureau,Shanghai 201202);Optimization of Growth Condition of Vibrio parahaemolyticus via Response Surface Methodology[J];Microbiology;2008-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Chao LI ChunZhen TAN TianWei (College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Optimization of glucose oxidase production by fermentation with Aspergillus niger using a response surface method[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2011-01
2 MAO Zhi-juan~1, LOU Dan~1,WU Jian-jun~2 (1.Ningbo Fisheries Research Institute, Ningbo 315012,China;2. Huazhong Agricultural University, Wuhang 430070,China);Studies on Culture Technological Conditions of Vibrio harveyi Y91301[J];Fisheries Science;2004-03
3 LIU Lian-sheng1,2,YAN Mao-cang1,ZHAO Hai-quan2,CHEN Qian-ying2,MA Ai-min1,3,LIN Zhi-hua11.Zhejiang Mariculture Research Institute,Wenzhou 325005,China;2.College of Life Science,Anhui Agricultural University,Hefei 230036,China;3.College of Animal Sciences and Technology,Shandong Agricultural University,Tai'an 271018,China);The Optimal Culture Conditions of Bacterium Vibrio harveyi WG1702 from Clam Meretrix meretrix[J];Fisheries Science;2010-02
4 YAO Dong-rui,ZHU Ming,CHEN Li,BI Ke-ran,SHI Ai-qin,ZHANG Xiao-junCollege of Ocean,Huaihai Institute of Technology,Jiangsu Marine Resources Development Research Institute,Lianyungang 222005,China);Development of PCR Method for Detection of Pathogenic Baterium Vibrio harveyi Based on ToxR Gene[J];Fisheries Science;2011-09
5 ZHANG Xiao-jun,BI Ke-ran,YAN Bin-lun,QIN Lei,LIANG Li-guo( College of Ocean,Jiangsu Key Laboratory of Animal Diseases in Mariculture,Huaihai Institute of Technology,Lianyungang 222005,China);Identification and PCR Detection of Pathogenic Vibrio harveyi Isolated from Gobby Synechogobius hasta[J];Fisheries Science;2011-12
6 GUI Miaomiao~(1,2) (1 College of Materials Science and Engineering,Tongji University,Shanghai 200092; 2 Xiamen Academy of Building Research(Group) Co.,Ltd,Xiamen 361004);Optimization Autoclaved Aerated Concrete Mortar Formula Using Response Surface Methodology[J];Materials Review;2010-S1
7 Du Guangyinghui,Cui Daizong,Zhang Ning,Zhao Min,Li Guofang,Gu Xiaoxu(College of Life Sciences,Northeast Forestry University,Harbin 150040,P.R.China);Optimization of Culture Medium Composition for Azoreductase Production by Escherichia coli CD-2 Using Response Surface Methodology[J];Journal of Northeast Forestry University;2012-05
8 LUO Dian-hui,WANG Wen-yan,YUE Jun-yang,FANG Bai-shan (Department of Bioengineering and Biotechnology,Huaqiao University,Xiamen 361021,China);Culture Conditions of Vibrio fischer[J];Environmental Science & Technology;2010-08
9 HANG Wan-hong,CHEN Yue-zhong(Abalone institute of Zhangpu county,Zhangpu 363205,China;Fisheries research institute of Fujian,Xiamen 361012,China);A Primary Study of Prevention and Cure of Penaeid Shrimp Luminous Disease by Using Biological Control Methods[J];Journal of Fujian Fisheries;2007-02
10 SHU Bo2,HUANG Degui1,GUAN Wanchun1,PAN Changwang1,CHANG Yanxiang1(1.Wenzhou Medical College,Wenzhou 325035,China;2.Hunan Yongqing Envirojnmental Conservation Ltd-Co,Changsha 410005,China);Impact of PFOS on Growth and ECP Gene of Vibrio harveyi[J];Guangdong Trace Elements Science;2011-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FANG Qin-mei LIN Tian-long GONG Hui YANG Jin-xian LIU Xiao-dong (Institute of Animal Husbandry and Veterinry Medicine,Fujian Academy of Agricultral Sciences Fuzhou Fujian 350003,China);Preparing the ISCOMs of Aeromonas hydrophila β-hemolysin expressed in E.coli[J];Fujian Journal of Agricultural Sciences;2004-04
2 XU Bin-fu,LIN Neng-feng,YU Fu-song,LIN Tian-long(Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian,350005,China);Immunological effect of OMP-ISCOMs from Pseudosciaena crocea on Vibrio alginolyticus[J];Fujian Journal of Agricultural Sciences;2008-03
3 Ma Yue, Zhang Yuanxing, Sun Xiuqin  (State key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237) ( First Institute of Oceanography, State Oceanic Administration, Qingdao 266003);Production of Marine Fish Vaccine for Vibriosis from Vibrio anguillarum: Optimization of New Culture Medium for High density Culture[J];High Technology Letters;2001-07
4 XU Bin-fu1,GONG Hui1,CHEN Xiu-jin2,TIAN Ding1,CHEN Qiang1,CHEN Yong-cong1,LIN Tian-long1(1.Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China; 2.Fujian Center for Disease Control and Prevention,Fuzhou,Fujian 350001,China);Minimum Dosage of Vibrio vulnifiticus OMP-ISCOMs for Vaccinating Anguilla anguilla[J];Fujian Journal of Agricultural Sciences;2012-12
5 SHI Zhu,LIU Qin,ZHANG Zhi-hui,YANG Min-jun,ZHANG Yuan-xing(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China);Evaluation on Biosafety of Attenuated Vibrio anguillarum Based on Module Animal Zebrafish[J];Journal of East China University of Science and Technology(Natural Science Edition);2011-06
6 GAN Ling-ling1,2 WANG Wei-fang2 LEI Ji-lin2* LIU Xin-fu2 WEN Hai-shen1 (1 Key Laboratory of Mariculture,Ministry of Education, Ocean University of China, Qingdao 266003) (2 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071);Fishery vaccine research and prospects in flatfish[J];Progress in Fishery Sciences;2013-02
7 Zuo Wengong;Qian Huaxin;Xu Yingfang;Du Shenying;Yang Xianle Changjiang Fisheries Institute;A CELL LINE DERIVED FROM THE KIDNEY OF GRASS CARP (CTENOPHARYNGODON IDELLUS)[J];Journal of Fisheries of China;1986-01
8 WU Zong-fu1,GONG Hui1,2,CHEN Hong-yan1,YANG Jin-xian1,LIU Xiao-dong1,LIN Tian-long1(1.Bio-technology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China;2.Aquatic Pathogen Collection Center of the Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China);Immune effects of the eel after immersed with ISCOMs containing recombinant β-hemA of Aeromonas hydrophila[J];Journal of Fisheries of China;2007-03
9 GONG Hui1,2,LIN Tian-long2,ZHANG Xiao-pei2,LUO Tu-yan3,WU Zong-fu2,SONG Tie-ying2,YANG Xian-le1(1.Aquatic Pathogen Collection Center of the Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China; 2.Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350001,China;3.Central Laboratory of Agricultural Sciences,Fuzhou 350001,China);The preparation of ISCOMs with aerolysin from Aeromonas hydrophilla and the immune efficiency in Anguilla anguilla[J];Journal of Fisheries of China;2009-02
10 Xu Shuying; Li Huanlin; Deng Guocheng and Jiang Lan(Pearl River Fisheries Research Institute,Guangzhou 510380);THE PREPARATION AND IMMUNE EFFECT OF ATTENUATEDLIVE VACCINE OBTAINED THROUGH CELL CULTUREFOR HEMORRHAGE OF GRASS CARP[J];JOURNAL OF FISHERIES OF CHINA;1994-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MAO Zhi juan 1,LIU Guo yong 2,CHEN Chang fu 2 (1 Ningbo Fishery Research Institute,Ningbo 315012; 2 Key Laboratory of Agricultural Microbiology, Ministry of Agriculture);Isolation and Identification of Pathogenic Bacteria Causing Ulcerosis in Large Yellow Croaker (Pseudosciaena crocea)[J];Journal of Anhui Agricultural University;2002-02
2 Ma Yue, Zhang Yuanxing, Sun Xiuqin  (State key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237) ( First Institute of Oceanography, State Oceanic Administration, Qingdao 266003);Production of Marine Fish Vaccine for Vibriosis from Vibrio anguillarum: Optimization of New Culture Medium for High density Culture[J];High Technology Letters;2001-07
3 Mo Zhaolan, Mao Yunxiang , Chen Shiyong, Zhang Zhendong, Zhang Peijun (Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071) ( College of Marine Life Sciences, Ocean University of Qingdao,;Classification and Molecular Biological Analysis of One Pathogenic Bacterium Associated with Hamorrhages of Cultured Flounder[J];High Technology Letters;2001-12
4 Chen Shiyong, Mo Zhaolan, Zhang Zhendong, Xu Yongli, Zhang Peijun (Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071);Purification and Characterization of a Virulent Factor in Extracellular Products of Vibrio Anguillarum[J];High Technology Letters;2002-08
5 WANG Guo liang, JIN Shan, XUE Liang yi, QIAN Yun xia Ningbo University ,Ningbo 315211, China;Studies on the Skin Ulcer Disease and the Pathogenic Bacteria of Lateolabrax japonicus in Marine Cage Culture[J];JOURNAL OF OCEANOGRAPGY OF HUANGHAI &BOHAI SEAS;2000-03
6 FANG Wen 1,WU Lei 1,GE Xin 2,ZHANG Ying 1,GAN Yi-ru 1 (1. School of Chemical Engineering, Tianjin University, Tianjin 300072,China; 2.Tianjin Medical University, Tianjin 300070,China);Study on Culture Technological Conditions of Klebsiella Pneumoniae[J];Chemical Industry and Engineering;2002-01
7 LI Jun; XU Huai-shu(College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003);ISOLATION AND BIOLOGICAL CHARACTERISTICS OF VIBRIO HARVEYI AFFECTING HATCHERY-REARED PENAEUS CHINENSIS LARVAE[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1998-04
8 YOU Feng; WANG Ke-ling, XIANG Jian-hai, XU Cheng, WU Su-qi(Institute of Oceanology, ne Chinese Acadeny of Sciences, Qingdao, 266071);BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.ET S.) IN THE COASTAL WATERS OF SHANDONG[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1999-02
9 XU Cheng, WANG Ke Ling, XU Yong Li, ZHANG Pei Jun (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);RECOMBINATION AND EXPRESSION OF PATERNAL GENE OF ISOZYMES IN GYNOGENETIC OLIVE FLOUNDER PARALICHTHYS OLIVACEUS[J];Oceanologia Et Limnologia Sinica;2002-01
10 WU Hou-Bo, PAN Jin-Pei (South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou, 510301);PURIFICATION AND CHARACTERIZATION OF THE EXOTOXIN Vm-Pm PRODUCED BY VIBRIO MIMICUS[J];Oceanologia Et Limnologia Sinica;2002-01
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