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《Biotechnology Information》 2002-03
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Research on the Standard Condition of RAPD Analysis in Pigs

Jiang Caode Deng Changyan Xiong Yuanzhu (Key Opening Laboratory of Pig Genetics and Breeding of Agricultural Ministry, Wuhan 430070)  
In order to research the relationship between genetic parents distant and heterosis in pigs, the authors probe to some essential factors affecting RAPD pattern, including template concentration and purity, primer concentration, dNTP concentration, Mg ++ concentration, different brand Tag polymerase, etc. The optimal condition of amplification for RAPD assay in pigs as follows: in 25μl reaction solution, containing 10×buffer 2.5μl,MgCl 2 1.75mmol/L,dNTP 0.25mmol/L,random primer 0.24μmol/L, DNA polymerase2U,BSA1μg/μl. The PCR profiles are min at 94℃ for 1 cycle followed by 1 min at 94℃,1min at 36℃,2min at 72℃ for 40 cycles and 10 min at 72℃ as final extension step.
【CateGory Index】: Q523
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