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《Biotechnology Bulletin》 2009-07
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Analysis of SSR Information in ESTs Resource of Tobacco(Nicotiana tabacum)

Hu Zhongyi Cai Liuti Chen Xingjiang (Tobacco Science Resource Institute of Guizhou Province,Guiyang 550003)  
In this research,242 683 expressed sequence tags(ESTs) were analyzed by software,indicating that 9 339 SSR were found in 211 728 non-redundant tobacco ESTs,and the average distance between SSR was approximately 14.21 kb,accounting for 4.41% of ESTs,including 216 kinds of repeat motifs were mined out.Trimeric repeats,accounting for 50.34%,was dominant in all SSR,followed by dimeric and mono-meric repeats,23.00% and 16.48%,while the frequency for other repeat type was below 5% each.The most abundant motif was A/T,accounting for 14.68% in all repeat types,followed by AT/TA,AG/TC,AAG/TTC,accounting for 10.49%,9.48%,6.85%,respectively.10 pairs of primers for EST-SSR randomly designed were subjected to PCR for DNAs from six tobacco varieties and one pair of primers showed polymorphisms.The result provided a base for the development and application of EST-SSR markers in tobacco.
【Fund】: 贵州省烟草专卖局项目(黔烟科2007-03)
【CateGory Index】: S572
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Chinese Journal Full-text Database 10 Hits
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6 HAI Sa~ ①② LI Jia-Le~ ① LIU Feng~① FENG Jian-Bin~①(①College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;② Institute of Fisheries Science, Xinjiang Uygur Autonomous Region, Wulumuqi 830000,China);Isolation of Microsatellite Loci from Perca schrenkii and its Universal in Related Species[J];Chinese Journal of Zoology;2009-01
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2 Carl Changchun Lin Guoping Wang Yi Huang Wenchang Zheng Li (Burley Tobacco Experimental Station of CNTC,Hubei Tobacco Research Institute,Hubei,Wuhan 430030);Application of Molecular Marker Technology in Genetics and Breeding of Tobacco[A];[C];2007
3 XIN Ya*,WANG Wei-Ke,RUAN Song-Lin,WANG Shi-Heng,MA Hua-Sheng Hangzhou Academy of Agricultural Sciences,hangzhou,310024;Clustering Analysis of Oyster Mushroom strains(Pleurotus geesteranus) Based on RAPD and EST-SSR Markers[A];[C];2008
4 LIU Yan-hua,MOU Jian-min,WANG Zhi-de,DAI Pei-gang(Tobacco Research Institute of CAAS,11,4th Road Keyuan longitude,Laoshan district,Qingdao,Shandong,266101);Advance of Molecular Marker Application in the Tobacco Genetic Breeding[A];[C];2007
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