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《Biotechnology Bulletin》 2009-07
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Analysis of SSR Information in ESTs Resource of Tobacco(Nicotiana tabacum)

Hu Zhongyi Cai Liuti Chen Xingjiang (Tobacco Science Resource Institute of Guizhou Province,Guiyang 550003)  
In this research,242 683 expressed sequence tags(ESTs) were analyzed by software,indicating that 9 339 SSR were found in 211 728 non-redundant tobacco ESTs,and the average distance between SSR was approximately 14.21 kb,accounting for 4.41% of ESTs,including 216 kinds of repeat motifs were mined out.Trimeric repeats,accounting for 50.34%,was dominant in all SSR,followed by dimeric and mono-meric repeats,23.00% and 16.48%,while the frequency for other repeat type was below 5% each.The most abundant motif was A/T,accounting for 14.68% in all repeat types,followed by AT/TA,AG/TC,AAG/TTC,accounting for 10.49%,9.48%,6.85%,respectively.10 pairs of primers for EST-SSR randomly designed were subjected to PCR for DNAs from six tobacco varieties and one pair of primers showed polymorphisms.The result provided a base for the development and application of EST-SSR markers in tobacco.
【Fund】: 贵州省烟草专卖局项目(黔烟科2007-03)
【CateGory Index】: S572
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Chuan-zhi1,LI Ai-qin1*,LI Chang-sheng1,LIU Bo2,XIA Han1,WANG Xing-jun1,JIA Wen-bin1(1.High-tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province,Jinan 250100,China;2.Anqiu City Agricultural Bureau,Anqiu 262100,China);Analysis of SSR Information in EST Resources of Goat[J];Shandong Agricultural Sciences;2009-06
2 GUO Da-long(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Development of the Microsatellite Primer in Plant[J];Journal of Anhui Agricultural Sciences;2007-18
3 HU Zhong-yi et al (Guizhou Tobacco Research Institute,Guiyang,Guizhou 550003);Application and Prospect of Molecular Markers in Tobacco Breeding[J];Journal of Anhui Agricultural Sciences;2007-25
4 LI Jian-min g et al (College of Agronomy, Northwest A & F University, Yangling,Shaanxi 712100);Studies on Genetic Diversity of Genomic-SSR and ESTJSSR Molecular Marker in Common Wheat[J];Journal of Anhui Agricultural Sciences;2007-26
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6 HAI Sa~ ①② LI Jia-Le~ ① LIU Feng~① FENG Jian-Bin~①(①College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;② Institute of Fisheries Science, Xinjiang Uygur Autonomous Region, Wulumuqi 830000,China);Isolation of Microsatellite Loci from Perca schrenkii and its Universal in Related Species[J];Chinese Journal of Zoology;2009-01
7 ZHAO Xue,CHANG Wei,HAN Yingpeng,and LI Wenbin* Soybean Research Institute,Key Laboratory of Soybean Biology of Chinese Education Ministry,Northeast Agricultural University,Harbin 150030,China;Review of the Methods for Developing SSR Molecular Markers[J];东北农业大学学报(英文版);2008-01
8 CHEN Xiang-yan1,LI Wei2,DAI Hai-ying2,ZHANG Li-feng2(1Office,Shandong Academy of Agricultural Sciences,Jinan 250100,Shangdong;2Crop Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China);Analysis of SSR Information in EST Resource of Soybean(Glycine max)[J];Soybean Science;2009-03
9 ZHANG Jian-ping1,WANG Bin2,ZHAO Li-juan3,WANG Li-min1,YANG Chong-qing2,XIE Rui-xia2,DANG Zhan-hai1(1 Institute of Crop Science,Gansu Academy of Agricultural Science,Lanzhou 730070,China;2 Agronomy College,Gansu Agricultural University,Lanzhou 730070,China;3 Institute of Bio-technology,Gansu Academy of Agricultural Science,Lanzhou 730070,China);Screen of SSR Markers from EST of Flax(Linum usitatissimum L.)[J];Acta Botanica Boreali-Occidentalia Sinica;2009-05
10 CHANG Yu-mei1, 2, KUANG You-yi1, LIANG Li-qun1, LU Cui-yun1, HE Jian-guo2, SUN Xiao-wen1, (1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. College of Life Sciences, Zhongshan University, Guangzhou 510275, China);Searching for Protein-coding Genes Using Microsatellites in Common Carp by Comparing to Zebrafish EST Database[J];Zoological Research;2008-04
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2 Carl Changchun Lin Guoping Wang Yi Huang Wenchang Zheng Li (Burley Tobacco Experimental Station of CNTC,Hubei Tobacco Research Institute,Hubei,Wuhan 430030);Application of Molecular Marker Technology in Genetics and Breeding of Tobacco[A];[C];2007
3 XIN Ya*,WANG Wei-Ke,RUAN Song-Lin,WANG Shi-Heng,MA Hua-Sheng Hangzhou Academy of Agricultural Sciences,hangzhou,310024;Clustering Analysis of Oyster Mushroom strains(Pleurotus geesteranus) Based on RAPD and EST-SSR Markers[A];[C];2008
4 LIU Yan-hua,MOU Jian-min,WANG Zhi-de,DAI Pei-gang(Tobacco Research Institute of CAAS,11,4th Road Keyuan longitude,Laoshan district,Qingdao,Shandong,266101);Advance of Molecular Marker Application in the Tobacco Genetic Breeding[A];[C];2007
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