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《Biotechnology Bulletin》 2011-06
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Construction of a Fosmid Library of Wuyiencin' s Producing Strain and Obtaining of the Library Probe

Tan Beibei Sun Lei Zhang Kecheng Yang Zhenjuan Wu Zhe Zhang Jun (State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,CAAS,Beijing 100193)  
Streptomyces ahygroscopicus var.wuyiensis was taken as the material.Its total genome was digested partially by enzyme Sau 3AⅠ,and 35-45 kb fragments was extracted.Then the extracted fragments were ligated to vector pCC1FOS.After packaged and transfected,the Fosmid library of Wuyiencin's producing strain was constructed.The titer of the Fosmid library was 8.8×10~5 CFU/mL. We picked 16 positive clones and digested with EcoRⅠand HindⅢ.As a result,the electrophoresis showed that the average sizes of the inserting DNA ligated to the vectors were greater than 35 kb,and the inserting rate was 100%.According to the biosynthetic gene cluster of Nikkomycin,polyoxin and macrolides antibiotics,we designed some specific primers to PCR using genome DNA as template for probe screening.The result showed that the primes designed by using PKS amplified DNA fragment about 1 693 bp which had high similarity with other biosynthetic genes of macrolides antibiotics.Construction of a Fosmid library of Wuyiencin's producing strain and obtaining of the library probe will make a foundation for cloning of biosynthetic genes of Wuyiencin.
【Fund】: 公益性行业科研专项资金项目(201103002);; 中国农业科学院植物保护研究所中央级公益性科研院所基本科研业务费专项资金项目(161014201 1004)
【CateGory Index】: S482.2
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Chinese Journal Full-text Database 1 Hits
1 WU Zhe1,ZHANG Jun1,2,SUN Lei1,LIU Yanyan1,GE Beibei1,ZHANG Kecheng1(1.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193;2.College of Plant Protection,Henan Agricultural University,Zhengzhou 450002);Identification of Antagonistic Strain 1706 and Its Effect on Strawberry Powdery Mildew[J];Chinese Journal of Biological Control;2013-03
Chinese Journal Full-text Database 10 Hits
1 LI Rong-jie(Anhui Fengyuan Group Limited Company,Bengbu 233010,China);Research Progress of Microbial Mutation Breeding Techniques[J];Journal of Hebei Agricultural Sciences;2009-10
2 Zeng Hongmei;Wuyiencin, a New Antibiotic for Controlling Crop Fungi Diseases[J];Fine and Specialty Chemicals;2003-Z1
3 HE Yun-long,ZHU Dong-qing,BAI Lin-quan,DENG Zi-xin (Key Laboratory of Microbial Metabolism,College of Life Science and Biotechnology,Shanghai Jiaotong University,Shanghai 200240,China);Cloning of Antibiotic Biosynthetic Gene Clusters from Genomic Library by Narrow-down Polymerase Chain Reaction[J];Journal of Shanghai Jiaotong University;2009-01
4 Huan Minghui1 Chen Fei1 Wu Hongyan1 Guan Yanli1 Wang Zhi1 Cheng Yongtao2 (1Liaoning Scientific Research Academy of Microbiology,Chaoyang 122000; 2Liaoning Province Chaoyang City Hqiene School,Chaoyang 122000);Construction and Initial Screening of Gene Library of the Streptomyces ahygroscopicus[J];Biotechnology Bulletin;2009-01
5 Tian Yunlong 1 Zhu Changxiong 1 Jiang Xiliang2 Guo Ping1 Liu Xue 1(1Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agricultural Science,Beijing 100081;2 Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081);The PCR-based Screening of the Cosmid Library from Streptomyces lavendulae var. hainanensis[J];Biotechnology Bulletin;2009-11
6 ZHAO Hong-yan1,LI Li2 ,HUAN Ming-hui2 ,CHEN Fei2,WU, Hong-yan2 (1. Shenyang Agric. Univ. Shenyang 110161; 2. Liaoning Acad. of Microbiol. Sci., Chaoyang 122000);Construction of Gene Library of Streptomyces ahygroscopicus neosubsp.wuzhouensis[J];Journal of Microbiology;2009-01
7 Nie Liping\ Zhang Jihui\ Tan Huarong (Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China) (Department of Biology,Liaoning Wormal University,Dalian 116029, China);STRUCTURE AND FUNCTION OF sanL GENE INVOLVED IN NIKKOMYCIN BIOSYNTHESIS OF STREPTOMYCES ANSOCHROMOGENES[J];Acta Microbiologica Sinica;2001-01
8 LI Li-hua~1,TIAN Yun-long~2,JIANG Xi-liang~2,WANG Li-qun~(1*),WU Yong-ying~(1,3),ZHU Chang-xiong~(2*)(1.Northeast agriculture university Harbin 150030 China;2.Institute of Agricultural Environment and Sustainable Development,CAAS,Beijing;100081;3.Institute of sugar industry,Harbin institute of technology,150086);Cloning and sequence analysis of the Zhongshengmycin Biosynthetic Gene zpsA[J];China Journal of Bioinformatics;2006-01
9 SUN Yan-zhong, ZENG Hong-mei, SHI Yi-ping, LI Guo-qing, WANG Xiao-qing(Institute of Plant Protection , Chinese Academy of Agricultural Sciences, Beijing 100094, China; Department of Plant Protection , Huazhong Agricultural University, Wuhan 430070, China);Inhibition of Botrytis cinerea by wuyiencin and variation of enzymic activities associated with disease resistance in tomato[J];Plant Protection;2004-06
10 SUN Yan-zhong 1,2, ZENG Hong-mei 1, SHI Yi-ping 1, LI Guo-qing 2 ( 1 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2 Department of Plant Protection, Huazhong Agricultural University, Wuhan, Hubei 430070, China);Mode of action of wuyiencin on Botrytis cinerea[J];Acta Phytopathologica Sinica;2003-05
Chinese Journal Full-text Database 10 Hits
1 XU Peng,ZHANG Qing-qing,TANG Wen-jing,LV Wen-wen(Engineering Technology Research Center of Microbial Fermentation. Anhui Polytechnic University,Wuhu 241000,China);Breeding Bacillus natto with high antibacterial activity by compound mutation protoplast[J];Journal of Anhui Polytechnic University;2011-04
2 JIN Yi-wei et al (Zhejiang Institute of Subtropical Crops,Wenzhou,Zhejiang 325005);Efficacy Experiment of 2% Wuyiencin Aqueous Solutions against Cucumber Powdery Mildew[J];Journal of Anhui Agricultural Sciences;2008-16
3 HE Xin et al (China Environmental Management College,Qinhuangdao,Hebei 066004);Research Progress in Antifungal Antibiotics[J];Journal of Anhui Agricultural Sciences;2010-30
4 CHEN Shi-tong et al(Institute of Edible Fungi,Yunnan Agricultural University,Kunming,Yunnan 650201);Present Research on Breeding Methods of Edible Fungi and the Problems in It as Well as the Prospects[J];Journal of Anhui Agricultural Sciences;2012-10
5 ZHANG Xin-hu,HE Jing,SHEN Hui-min (College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,China);Primary study on fungistasis and fungistatic mechanisms of Xanthium sibiricum on Botrytis cinerea[J];Acta Prataculturae Sinica;2008-03
6 WANG Tie-feng1,MAO Ran1,ZHAO Xue-feng2,QIAN Ai-dong1(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin,130118,China;2.College of Life Science,Jilin Agricultural University,Changchun,Jilin,130118,China);Construction of DNA Library of a Mycobacterium Fortuitum Strain[J];Progress in Veterinary Medicine;2011-04
7 WANG Xing-quan,ZHANG Xin-hu,YANG Shun-yi,SHEN Hui-min (College of Prataculture,Key Laboratory of Grassland Ecosystem,Ministry of Education,The Sino-U·S Centres for Grazingland Ecosytem Sustainability,Gansu Agricultural University,Lanzhou 730070,China);The study on the action mechanism of the extraction from Xanthium sibiricum to Botrytis cinerea Pers[J];Journal of Gansu Agricultural University;2008-03
8 LUO Jian-jun,LI Hui,GENG Peng,HU Mei-ying(Key Laboratory of Natural Pesticide and Chemical Biology,Ministry of Education,South China Agricultural University,Guangzhou,Guangdong 510642 China);Isolation and identification of actinomyce strain MY-4 and its antagonistic activity against pathogenic fungi of fruits and vegetables[J];Journal of Fruit Science;2012-04
9 ZHANG Shu-qin,SHI Shang-li,CHEN Li-yu,MIAO Yang-yang, LI Jian-feng,HUO Ping-hui(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China);The effect of LaCl_3,IAA and plant sap on the growth and proliferation of fluorescence marked rhizobia[J];Grassland and Turf;2013-02
10 YU Wen-bin;WU Yu-huan;ZHANG Yan-jun;XIE Ming;Zhangjiakou Academy of Agricultural Sciences;Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Hebei North University;;Control Efficacy of Wuyiencin and Bacillus subtilis on Tomato Gray Mould Disease in Greenhouse[J];Northern Horticulture;2013-17
China Proceedings of conference Full-text Database 1 Hits
1 SHI Yi-ping ZENG Hong-mei (Institute of Plant Protection,CAAS,Beijing 100094);Manufacture and appliction of wuyiencin[A];[C];2004
Chinese Journal Full-text Database 10 Hits
1 WANG Zhong-wu,WANG Nan(Jilin Agricultural Science and Technology College,Jilin,Jilin 132101);Selection of Medicaments for Controlling Powdery Mildew of Strawberry Planted in Protected Field[J];Northern Horticulture;2010-19
2 LI Yanping XU Yang RUAN QiongfangWEI Kangxia GUO Liang YAN Shaohua(Sino-Germany Joint Research Institute,Key Laboratory of Food Science of Ministry of Education,Nanchang University,330047,Nanchang,China);CONSTRUCTION AND ANALYSIS OF A MONASCUS FOSMID LIBRARY[J];Journal of Beijing Normal University(Natural Science);2008-02
3 ZENG Zhi-hong(Department of Environment and Biological Science,Putian University,Putian 351100,China);A Brief Introduction to the Methods of Antibiotic Biosynthetic Gene Clusters Cloning[J];Journal of Changchun Normal University;2007-06
4 WEI Yan min, LIU Da qun, TIAN Shi min, ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Baoding 071001, China)[HJ4/9];The antagonism of 26 strains of Streptomyces spp. against several vegetable pathogens[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;2000-03
5 WANG Jiakun1 AN Peipei1 CHEN Zhenming2 YE Jun’an1 LIU Jianxin1(1. Institute of Dairy Sciences,Key Laboratory for Molecular Animal Nutrition of Ministry of Education,Zhejiang University,Hangzhou 310029,China; 2. Center for Biomedicine and Health,Hangzhou Normal University,Hangzhou 310012,China);Construction and Analysis of Fosmid Library of Rumen Microbiota of Hu Sheep[J];Chinese Journal of Animal Nutrition;2010-02
6 GAN Shun, LIAO Wen-hao, DU Zhong-you(Guizhou Guiyang National Agricultural Sciences & Technology Zone, Guiyang 550018, China);Control Effects of 0.05% Juntunong Emulsion on Strawberry Powdery Mildew[J];Guizhou Agricultural Sciences;2005-02
7 JIANG Yu~1,DONG Huai-yu~1,XU Xiu-de~1,LIU Zhi-heng~1,FAN Hui-mei~2(1.Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161;2.Shenyang Agriculture University,Shenyang,Liaoning 110161,China);Progress on the Research of Actinomycetes in Biocontrol of Plant Disease[J];Rain Fed Crops;2005-05
8 LI Bing1,YAN Shou-qing1,SUN Jin-hai1,2(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China);Construction of Fosmid Library in Domestic Pig[J];China Animal Husbandry & Veterinary Medicine;2007-10
9 Li Lijuan 1,2 Cai Jing 3 Zhang Shilai 2 Wang Li 1 Hu Fengyi 21 Yunnan Agricultural University,Kunming,650231;2 Food Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,650205;3 Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,650223;Whole Genome Fosmid Library Construction of Wild Rice(Oryza longistaminata)[J];Molecular Plant Breeding;2012-04
10 MA Li~1,ZHANG Li-ping~2, ZHAO Gang-yong~2 (1.North China Petroleum Hygiene School, Renqiu Hebei 062552, China; 2.College of Life Science, Hebei University, Baoding Hebei 071002, China);The method of actinomycetes polyphasic taxonomy[J];Journal of The Hebei Academy of Sciences;2004-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Li et al (Biology Pesticide Staff Room,Shenyang Agricultural University,Shenyang,Liaoning 110161);Studies on Mutation Breeding in Producing Strain of Streptomycin avermitilis by the Irradiation of Co~(60)γ Ray[J];Journal of Anhui Agricultural Sciences;2007-13
2 ZHAO Li-kun et al (College of Life Sciences,Hebei University,Baoding,Hebei 071002);Research on the Mutation Breeding of Avermectin High-producing Strain[J];Journal of Anhui Agricultural Sciences;2008-19
3 SHI Yi-jun et al(College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou,Zhejiang 310036);Study on Mutation Breeding of Trichodermin Producing Strain[J];Journal of Anhui Agricultural Sciences;2008-29
4 Du Run-pan Li Xue-kang Ye Wen-zhong Wu Shang-zheng;MUTAGENIC BREEDING OF Streptomyces griseus WITH FAST NEUTRONS[J];Journal of Fudan University(Natural Science);1981-04
5 JIANG Shichun WU Jianping BAI Hua (Zhejiang HISUN Pharmaceutical CO.LTD .Taizhou 318000);SELECTION OF DAUNORUBICIN-PRODUCING STRAIN S.COERULEORUBIDUS BY PLASMA RADIATION TECHNOLOGY[J];Journal of Radiation Research and Radiation Processing;2001-02
6 Xu Junwang, Zhu Zhen, Li Xugang (Institute of Genetics, Academia Sinica, Beijing 100101);Isolation and Identification of Starch Synthase cDNA Using a Simple Procedure Based on PCR[J];High Technology Letters;2001-08
7 Zhu Jianlan(Department of plant Protection, Gansu Agricultural University, Lanzhou 730070);The Biological Characteristicsof Tomato Grey Mould Pathogen[J];JOURNAL OF GANSU AGRICUTURAL UNIVERSITY;1995-01
8 Zha Dongxing;Tang Jiliang;Ma Qingsheng(Laboratory of Molecular Genetics,Guangxi Agric.Univ.,Nanning 530005);Evidence of Exopolysaccharide-deficient Mutants Derived from Transposon Mutagenesis of Xanthomonas campestris pv.campestris[J];;1996-04
9 WEI Jun~1,JING Jian-zhou~1,SONG De-gui~2 (1.Institute of pood and Biology Engineering,Zhengzhou Institute of Light Industry,Zhengzhou 450002,China;2.College of Life Science,Guangxi Normal University,Guilin 541004,China);Mutant Breeding L-argining-producing Bacterium by N~+ Beam[J];Journal of Guangxi Normal University(Natural Science Edition);2006-02
10 Guo Ailian,Xu Jingui,Yang Lin School of Life Science,Northwest University,Xi′an 710069,China;He-Ne LASER BREEDING OF THE WHITE ROT-FUNGUS L_x WITH HIGH LIGNIN DEGRADATION RATE[J];Acta Photonica Sinica;2001-06
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