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《Biotechnology Bulletin》 2013-09
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Genetic Diversity of Wild Populations of Conyza blinii Lévl. and Extraction of Total Flavonoids

Liu Shan;Sun Rong;Tang Zizhong;Gao Jinglei;Hu Hongli;Chen Hui;College of Biology and Science,Sichuan Agricultural University;Department of Biological and Chemical Engineering,Panzhihua University;  
The genetic relationship of Conyza bliniiLévl.was analyzed in molecular level and the distribution characteristics of total flavonoid constituents.The RAPD method was applied to the study onConyza bliniiLévl.from different germplasms by use of 10 random primers about 10 bp,and the parameters were calculated by NTSYSpc2.1 and the relationship was constructed based on UPGMA.Use UV spectrophotometry to test total flavonoids in each sample.10 primers screened out from 100 primers were used for RAPD amplification.A total of 71 bands were generated,of which 68 bands were polymorphism bands.According to RAPD analysis,18 germplasms ofConyza bliniiLévl.were classified into two categories.The genetic diversity of the 18 species inConyza bliniiLévl.is high and the total flavinoids was related of geographic distribution.
【Fund】: 四川省科技创新苗子工程资助项目(2012ZZ046);; 攀枝花学院校级科研项目(2012YB03)
【CateGory Index】: Q943;S567.239
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