Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《BIOTECHNOLOGY INFORMATION》 1999-01
Add to Favorite Get Latest Update

The Difficulties in the Isolation of RNA from Plant Tissues and their Resolving Strategies

Li Hong 1,2 Wang Xinli 1 1 Laboratory of Plant Biotechnology, Institute of Micobiology, Chinese Academy of Sciences, Bejing, 100080; 2 Department of Light Industry, Hebei Sciences and Technology University, Shijiazhuang, 050018  
Phenolic compounds, polysacchrides, proteins and unidentified secondary metabolites are four main factors interfering the isolation of RNA from plant tissues. We described here that their interference, phenomena and methods for eliminating them.
【CateGory Index】: Q522.03
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 9 Hits
1 CHEN Xiao-feng;ZENG Qi-feng;DENG Xu;ZENG Guang-yao;ZHOU Ying-jun;Xiangya School of Pharmaceutical Science,Central South University;;A Method for Extracting Total RNA of Taxus chinensis at Large-scale[J];生物技术通报;2017-11
2 Cui Bo;Wang Ruolan;Zhang Yan;Liang Fang;Jiang Suhua;School of Life Sciences, Zhengzhou University;Bioengineering Research Institute, Zhengzhou Normal University;;Comparisons of Extraction Method of Total RNA from Paeonia suffruticosa Seeds[J];分子植物育种;2017-05
3 Yin Desong;Song Jingwu;Qi Beibei;Chen Yingying;Zhou Ling;Zhao Kai;Peng Lei;College of Horticulture and Landscape, Yunnan Agriculture University;College of Longrun Pu-erh Tea, Yunnan Agricultural University;;Effect of β-mercaptoethanol on Total RNA Quantity of Mango Pruning Wound Buds in Different Periods[J];中国农学通报;2017-10
4 DENG Jiao;Research Center of Buckwheat Industry Technology,Guizhou Normal University;Wuhan Botanical Garden,Chinese Academy of Sciences;;A simple method of extracting the total RNA from lotus petals[J];贵州师范学院学报;2017-03
5 SHEN Yan;LIU Huan;MA Hua-qing;CHEN Ping;Key Laboratory of Tropical Crop Germplasm Resources Protection and Genetic Improvement,Ministry of Education/College of Horticulture and Landscape Architecture,Hainan University;;Comparison of Several Methods for RNA Extraction from Wax Apple[J];江西农业学报;2017-03
6 GAO Xiang-feng;WANG Rang-jian;LIU Feng-jing;LI Hui-ling;Tea Institute,Fujian Academy of Agricultural Sciences;;Hydroponic Culturein Sample Preparation of Tea Shoots for RNA Sequencing[J];福建农业学报;2017-02
7 NIU Mei-yun;CHENG Qing-wei;ZHANG Yue-ya;ZHANG Xin-hua;MA Guo-hua;South China Botanical Garden,Chinese Academy of Sciences;University of Chinese Academy of Sciences;;Comparison of Several RNA Extracting Methods from Heartwood of Santalum album[J];热带亚热带植物学报;2017-01
8 GAO Tian;TIE Ying;WANG Yan;QI Zhi;College of Life Science, Inner Mongolia University;Inner Mongolia Hesheng Ecological Science and Technology Research Institute Company Limited;;Construction of c DNA library induced by low Ca~(2+)in Arabidopsis thaliana[J];北方农业学报;2016-05
9 Da Guozheng;Yang Min;Huang Jing;Shang Xiangwei;Zhang Xiuqiao;College of Pharmacy,Hubei University of Chinese Medicine;;Comparison of the RNA Extraction Effect from Different Tissues of Ampelopsis megalophylla[J];基因组学与应用生物学;2016-10
China Proceedings of conference Full-text Database 5 Hits
1 QIU Shuo XIONG Lei QU Yuan WU Jia LIU Zhang-dong WANG Cai-yun (College of Horticulture and Forestry Sciences,Key Laboratory of Horticultural Plant Biology, Ministry of Education,Huazhong Agricultural University,Wuhan 430070);Comparison of Different RNA Isolation Protocols of Mature Leaves of Some Dendrobium Species[A];[C];2012
2 REN Hong-yan CHENG Kai ZHENG Cheng-shu (College of Horticulture Science and Engineering,Shandong Agricultural University /State Key Laboratory of Crop B iology,Tai'an 271018);Establishment and Application of cDNA-AFLP Analysis System of Chrysanthemum[A];[C];2011
3 Zhang Huping~1 Niu Jianxin~1 Wang Guo'an~2 Hu Haifang~2 Ma Binggang~1 Zhu Jun~1 (~1Department of Horticulture.College of Agriculture.Shihezi University,Shihezi 832003,China;~2 Xinjiang Forestry Academy.Urumqi 830000,China);Establishment of Optimal Reaction System of RAPD in Walnut[A];[C];2003
4 GUAN Cui-ping~1 ZHANG He-ling~2 MEN Fu-yi~1 SONG Yan-ru~3 MENG Mei-lian~1 CHEN Zheng-hua~4 1.Inner Mongolia Agricultural University 010018; 2.Inner Mongolia University 010022; 3.Institute of Botany,CAS 100093; 4.Institute of Genetics,CAS 100101;A STUDY ON THE ONE-STEP RT-PCR FOR DETECTION OF POTATO VIRUSES[A];[C];2001
5 XU Qiu-hong,GAO Zhi-hong,QU Shen-chun,and QIAO Yu-shan~* (College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China);Effect of Sorbitol on Total RNA Isolation from Plum Fruit Flesh[A];[C];2009
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Kang Yaxuan;Chen Wu;Pang Xiaoming;Li Yingyue;National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plant, Ministry of Education, Beijing Forestry University;;The Comparison and Improvement of RNA Extraction Methods in Different Tissues in Chinese Jujube(Ziziphus jujuba Mill.)[J];分子植物育种;2015-12
2 Zhang Qing;Liu Shuai;Xing Yu;Cao Qingqin;Qin Ling;Beijing Key Laboratory of New Technology in Agricultural Application, Beijing University of Agriculture;;Comparison of Extraction Methods of Total RNA from Strawberry Fruits[J];中国农学通报;2015-31
3 JIANG Suhua;LI Yanhui;WANG Jieqiong;LIANG Fang;WANG Mofei;CUI Bo;Institute of Biotechnology,Zhenzhou Normal University;Fengqiu Bureau of Agriculture;College of Life Science,Henan Agricultural University;;Comparison research of extraction method of total RNA from sedirea japonica[J];河南农业大学学报;2015-04
4 LI Yun-he;ZHANG Zhi;WU Qing-song;South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture;;Isolation and Expression Analysis of an Ethylene Receptor Gene Mi ETR1b in Mango[J];园艺学报;2015-06
5 WEI Junya;TANG Jie;LIU Guoyin;LIU Debing;CHEN Yeyuan;Tropical Crops Genetic Resources Institute/Chinese Academy of Tropical Agriculture Sciences;Agriculture College,Hainan University;Applied Science and Technology College,Hainan University;;Cloning and Expression Analysis of MSOC1 Gene in Mango(Mangifera indica L.)[J];西北植物学报;2015-06
6 Zhang Bo;Zhao Zhichang;Gao Aiping;Chen Yeyuan;Huang Jianfeng;Dang Zhiguo;Luo Ruixiong;College of Agriculture Hainan University;Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China;;Cloning and Expression Analysis of DFR Gene from Mango(Mangifera indica)[J];分子植物育种;2015-04
7 Zhang Yanlong;Han Xueyuan;Niu Lixin;Zhang Jing;He Lixia;College of Forestry,Northwest A&F University;College of Horticulture,Northwest A&F University;Gansu Forestry Science and Technology Extend Station;;Analysis of Fatty Acid in Seed Oil from Nine Wild Peony Species[J];中国粮油学报;2015-04
8 ZHANG Tao;GAO Tianshu;BAI Ruiying;HUANG Xinglin;GUO Nan;LU Junxing;LUO Ancai;College of Life Sciences,Chongqing Normal University;;Utilization and Research Progress of Oil Tree Peony[J];重庆师范大学学报(自然科学版);2015-02
9 DONG Long;LUO Cong;HE Xinhua;HU Ying;YU HaiXia;College of Agronomy,Guangxi University;Guangxi Crop Genetic Improvement and Biotechnology Lab;;Cloning and Expression Analysis of a Flavonoid Sulfotransferases Gene from Mango(Mangifera indica)[J];热带作物学报;2014-04
10 ZHANG Xiao-li;DAI Hong-jun;Agricultural College,Ningxia University;;Research Progress on Extraction Method of Plant RNA[J];北方园艺;2014-08
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LIU Yang;TIE Ying;YANG Jia;QI Zhi;MAO Huiping;Inner Mongolia Hesheng Ecology Science and Technology Research Limited;College of Life Sciences,Inner Mongolia University;;Mongolian Almond Ca~(2+) Binding Protein Encoding Gene Systemic Identification and Expression Analysis[J];华北农学报;2017-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved