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《Acta Biologiae Experimentalis Sinica》 1989-04
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STUDIES ON DNA METHYLATION IN TRANSFORMED MOUSE LIVER CELLS

He Zhong-xiao Xue Ji-yan and Wu Guo-li (Laboratory of Molecular Biology and Biochemistry,Beijing Normal University;National Laboratory of Biomacromolecules,Academia Sinica;Beijing,China)  
Activity of DNA methylase and DNAmethylation level were measured from normalmouse liver,mouse liver charged with H_(22a)ascitic hepatoma and H_(22a) ascitic hepatomacell by measuring incorperation of H~3-methyl.S-Adenosyl-~3H-methyl-methionine (~3H-SAM)was used as methyl donor.DNA methylationlevel of different cells were measured by HP-LC.DNA methylase activity and DNA methylation level of H_(22a) ascitic hepatoma,mouseliver charged with H_(22a) ascitic hepatoma arelower than normal mouse liver.Treatmentsof antitumor drugs lead to a rising of DNAmethylase activity of tumor cell,however,the DNA methylation level of tumor cell hasnot rised after such treatments.
【Fund】: 国家自然科学基金(高技术)资助项目。项目编号:0388011
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