GENETIC DIVERSITY OF YAM (DIOSCOREA OPPOSITA THUNB) DETECTED BY ISSR MARKERS
ZHOU Yan Qing;JING Jian Zhou;LI Zhen Yong;HAO Jian;JIA Jing Fen;ZHANG Bao Hu;HAO Jian Guo College of Life Sciences, Northwest University, Xi'an 710069, China Postdoctoral Programme, Sino-American Biotechnology Company, Luoyang 471003, China Department of Biological and Biotechnology, Tsinghua University, Beijing 100084, China College of Life Sciences, Henan Normal University, Xinxiang 453007, China Wenxian Institute of Agricultural Sciences, Wenxian 454881, China
Genetic diversity of 28 cultivars of yam (Dioscorea opposita Thunb) was assessed by means of Inter-simple sequence repeat (ISSR) markers. The results showed that seven proper primers, with rich polymorphism, could be selected from a total of forty four ISSR ones; distinct differences appeared among 28 cultivars amplified bands, and the rate of polymorphic bands was 83.01%;Shannon's Information index was 0.3191; a Jaccard's genetic similarity matrix and a den- drogram for these cultivars were formed, in which they could be divided into four groups: Groupl was composed of D.opposita.cv. Ribenbai, D.opposita.cv.Huashanyao and D.opposita.cv.Ribenyuan; Group2 contained D.opposita.cv. Xiaoye; Group3 contained D.opposita.cv. No.1 Songye; other 23 cultivars were put into Group4. PCA(Principal component analysis) was employed to evaluate the resolving power of the markers to differentiate among them. This laid the foundation of the identi- fication of yam cultivars and the efficient use of its germplasm resources.
【Key Words】：
Dioscorea opposita Thunb ISSR marker Cultivar identification Genetic diversity
【Fund】： 国家自然科学基金（30370697）
【CateGory Index】： S632.1;
【Fund】： 国家自然科学基金（30370697）
【CateGory Index】： S632.1;
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