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《Journal of Molecular Cell Biology》 2006-05
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ISOFLAVONE ACCUMULATION ASSOCIATED WITH CELL STRUCTURAL CHANGES IN MAACKIA AMURENSIS SUSPENSION CULTURES ELICITED BY METHYL JASMONATE,SALICYLIC ACID AND NITRIC OXIDE

LUO Jian Ping XIA Ning SHEN Guo Dong (School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009)  
Comparison between changes in isoflavone accumulation and cell structure in Maack-ia amurensis suspension cultures elicited by methyl jasmonate (MeJA), salicylic acid (SA) and nitric oxide (NO) was studied. The results suggested that MeJA,SA and NO can all stimulate isoflavone production remarkably. After 9d treatment with 200μmol/L MeJA, 100μmol/L SA and 50μmol/L SNP,the isoflavone content was 417.18%, 185.45% and 222.45% of the control,respectively. At the same time,the electron-dense body (EDB) could be easily found in the cells,while its number achieved most after 9 d treatment,and the number of EDB and isoflavone content were positive correlated. It was deduced that elicitors stimulated plant secondary metabolites production associated with changes in cell structure.
【Fund】: 国家自然科学基金(项目编号:30170059)。~~
【CateGory Index】: Q946;S567.19
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