Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Letters in Biotechnology》 2001-01
Add to Favorite Get Latest Update

Making lung cancer related cDNA microarray

FAN Bao?Xing,ZHANG Kai?Tai,MA Shu?Hua,XIE Ling,WANG Sheng?Qi,WU De?Chang (Institute of Radiation Medicine,Beijing 100850,P.R.China)  
This study aimed at introducing a method of making lung cancer related cDNA Microarray and its using in gene expression profile. 60 lung cancer related genes were cloned, purified and micro?arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from passage 0, 20, 35 in malignant transformation cell model was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA. After scanning using ScanArray 3000, It showed analysised pots with uniformity background and clear signal.
【Key Words】： cDNA microarray BEP2D cell gene expression profile
【Fund】： 国家重点基础研究发展规划 973资助项目 (G19980 5 12 0 8)
【CateGory Index】： Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 5 Hits
1 BIN Xiaonong, LU Jiachun, TAN Min, JI Weidong, CHEN Jiakun. Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou, Guangdong 510182, P.R.China;Study on gene expression pattern in 16HBE cells treated with NiS[J];Chinese Journal of Lung Cancer;2005-05
2 FAN Bao-xing 1 ,SUN Jing-fen 2 ,LIU Qing-feng 1 ,CHEN Liang-an 1 ,LIU You-ning 1 ,WANG Sheng-qi 2 ,WU De-chang 2 1.Department of Respiratory Disease,General Hospital of PLA,Beijing100853;2.Beijing Institute of Radiation Medicine,Beijing100850,China;The diverse research of the different probes when hybridize with the cDNA microarray which come from total RNA and mRNA[J];Letters in Biotechnology;2004-01
3 HAN Jin-xiang, GAO Xue-qin . Shandong Medicinal Biotechnological Center , Shandong Academy of Medical Sciences , Jinan 250062, China;Comments on the Molecular Biology of Cancer Research in China[J];Journal of Qilu Oncology;2003-01
4 BIN Xiaonong,TAN Min,L Jiachun,et al.Institute for Chemical Carcinogenesis,Guangzhou Medical College(Guangzhou 510182,China);Gene chip screen for gene expression pattern in 16HBE cells treated with BPDE[J];Chinese Journal of Public Health;2006-11
5 Fan Baoxing, Da Jiping, Zhang Kaitai, Xie Ling' Xiang Xiaoqiong, Wang Shengqi, Wu Dechang ( Department of Molecular Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China; Department of Pathology, Xi Diao Yu Tai Hospital, Beijing 100036, P. R. China);Screen the lung cancer related diagnosis genes[J];The Journal of Evidence-based Medicine;2002-02
China Proceedings of conference Full-text Database 1 Hits
1 BIN Xiaonong,TAN Min,LU Jiachun,et al. Institute for Chemical Carcinogenesis,Guangzhou Medical College, Guangzhou 510182 P.R.China;Study on differentially gene expression pattern in 16HBE cells treated with BPDE[A];[C];2006
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HUO Yan-ying,ZHANG Kai-tai,LI Bang-yin,DUAN Rui-feng,FAN Bao-xing,XIANG Xiao-qiong,HU Ying-chun,XIE ling,WU De-chang Department of Molecular Toxicology,Beijing Institute of Radiation Med icine,Beijing 100850,P.R.China;Regulation of Smad7 Gene by TGF-β1 in Process of Malignant Transformation[J];Chinese Journal of Cancer;2002-02
2 CHEN Shao-Quan1, CHEN Ju Xiang 2, SHI Jing Hua 1, HU Zhi Qian 1, YING Kang 3, TANG Rong 3, LI Yao 3, FU Wei 4, XIE Yi 3* , MAO Yu Min 3* (1. Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shangh;Screening differentially expressed genes in gastric adenocarcinoma by cDNA microarray[J];Academic Journal of Second Military Medical University;2001-06
3 ZHENG Jie, FANG Wei Gang, WU Bing Quan (Department of Pathology, Peking University School of Basical Sciences, Beijing 100083, China);Biological characteristics of metastatic cancer cells and cloning of human cancer metastasis-related genes[J];Journal of Beijing Medical University;2001-05
4 YANG Zhi-lin1, XU Ru-xiang1, DU Mou-xuan1, ZOU Yu-xi1, CAI Ying-qian1, JIN Ying2, HU Geng-xi2 (1Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China; 2Max-Plank Guest Laboratory, Institute of Cell Biolo;Study on the differential gene expression profile of glioblastoma with microarrays[J];Journal of First Military Medical University;2001-08
5 YANG Shao-bo WANG Meng-wei YOU Wei-di LU Ying-lin CHEN Kun (Dep artment of Gastroenterology, 304th hospital of the PLA Beijing, China 100037);DETECTION OF GENE EXPRESSION IN HEPATOCELLULAR CARCINOMA BY IN SITU HYBRIDIZATION WITH PHOTOBIOTIN-LABELLED PROBE[J];CHINESE JOURNAL OF CANCER;1995-05
6 JI Wei-dong, WU Zhong-liang, CHEN Jia-kun(Institute for Chemical Carcinogenesis of Guangzhou Medical College, Guangzhou 510182, China);MALIGNANT TRANSFORMATION OF HUMAN BRONCHIAL EPITHELIAL CELL LINE INDUCED BY CRYSTALLINK NICKEL SULFIDE[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-01
7 WU Xiao-hua, XU Xiao-hu, SHEN Zhong-ying, et al. (Department of Forensic Pathology, Department of pathology, Shantou University Medical College, Shantou 515031, P. R. China);Expression of cell-cycle regulators cyclin D1 and CDK4 in esophageal squamous cell carcinoma[J];CHINESE JOURNAL OF CANCER;2000-01
8 LI Sheng,SHI Xue tao,ZHANG Xin,et al. Shandong Tumor Hospital,Jinan 250117;The relationship between Bcl-2,apoptosis and c-myc in pancreatic cancer[J];Journal of Hepatopancreatobiliary Surpery;2001-04
9 TANG Rong, YING Kang 1,CHEN Xuan mao 1,SUN Zhao hui 1,SHI Jun 2, WU Chao qun 1, HUANG Yan 1,LIU Jian ping 1, Li Yao, XIE Yi 1,MAO Yu min 1 (1.Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China; 2.Depar;Expression Pattern and Gene Clustering Analysis of Human Casein Kinase CK1 γ1 cDNA by Using cDNA Microarray Hybridization[J];Journal of Fudan University;2000-06
10 PU Yi ping,WU Jia lian,CAO Qian, et al (Department of ENT, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010);Influence of antisense oligodeoxynucleotide targeting against telomerase on expression of carcinoma gene of laryngeal carcinoma cells[J];Chongqing Medical Journal;2002-01
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 Liang Li,Ding Yan-Qing ,Shi Yi-Min;Suppression Subtractive Hybridiza tion and Its Application in Study of Tumors[J];Chinese Journal of Cancer;2003-09
2 AO Lin 1 ,GAO Li-hong 1 ,HU Ran 1 ,ZENG Zhi-xiong 2 , FANG Zhi-jun 2 ,CAO Jia 1, ,YANG Meng-shu 2 (1.Department of Toxicology,College of Preventive Medicine, Third Military Medical University,Chongqing400038,China; 2.Applied Research Center for Genomic Technology,City University of Hong Kong,Hong Kong,China);The Validation of Mouse Toxicology Gene Chip Technology[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-02
3 ZHENG Miao,LIU Wen-li(Dept.Hematology,Tongji Hospital,Tongji Medical College,Huazhong Science and Technology University,Wuhan 430030,China);Progress in the application of suppression ubtractive hybridization[J];Basic & Clinical Medicine;2006-11
4 HUANG Yu-ai,WANG Fei,LU Jing (Department of Food Quality and Safety, College of Quartermaster Technology, Jilin University, Changchun 130062, China);Application of Cell Culture Technique on Rapid Toxicity Evaluation in Food Toxicology[J];Food Science;2007-08
5 CHEN Zhao-li,LI Hai-feng,WANG Jing-feng,et al.Institute of Environment and Health,Academy of Military Medical Science(Tianjin 300050,China);Screening of lung tumor susceptibility gene caused by benzo(a) pyrene dihydrodiol epoxide[J];Chinese Journal of Public Health;2008-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved